RT info:eu-repo/semantics/doctoralThesis
T1 Búsqueda de biomarcadores de actividad inflamatoria en uveítis como posible diana terapéutica
A1 García Onrubia, Luis
A2 Universidad de Valladolid. Escuela de Doctorado
K1 Oftalmología
K1 Uveitis
K1 Uveítis
K1 Biomarker
K1 Biomarcador
K1 Cytokine
K1 Citocinas
K1 Impression cytology
K1 Citología de impresión
K1 32 Ciencias Médicas
AB Uveitis is an inflammation of the uvea that can lead to irreversible vision loss if not properly diagnosed, managed, and treated. Given the complexity of its diagnosis and the challenges in therapeutic management, there is growing interest in using biomarkers to enable precision medicine.This thesis analyses the concentration of cytokines and chemokines in the tear film, as well as the gene expression profile in the conjunctival epithelium of patients with uveitis and its evolution throughout the disease. Its aim is to identify biomarkers of inflammatory activity in uveitis patients.Two observational, prospective, and analytical case-matched studies were conducted on patients treated at the Hospital Clínico Universitario de Valladolid and the Instituto Universitario de Oftalmobiología Aplicada to achieve this. In the first study, cytokine levels in the tear film and plasma were analysed during both the active and inactive phases of the disease, revealing significant differences in the detection rate of IL-6. However, no differences were observed in its concentration. In the second study, the gene expression of 40 inflammatory molecules in conjunctival cells was evaluated, demonstrating differences in the detection of fractalkine, IL-6R, NOD2, TGF-β1, TLR4, and TNF-α between the active and inactive phases.The results suggest that the cytokine and chemokine expression profile on the ocular surface may serve as a useful marker for assessing inflammatory activity in uveitis and could potentially be a therapeutic target. Additionally, differences in gene expression were found according to HLA-B27 status, which could aid in the development of personalised therapeutic strategies.
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/76278
UL https://uvadoc.uva.es/handle/10324/76278
LA spa
NO Escuela de Doctorado
DS UVaDOC
RD 08-jul-2025