RT info:eu-repo/semantics/article T1 Viral RNA reduction from wastewaters using microalgae-based treatments: Elucidating the effect of light and zero-valent iron nanoparticles A1 Torres Franco, Andrés Felipe A1 Leroy Freitas, Deborah A1 García Encina, Pedro Antonio A1 Muñoz Torre, Raúl K1 E. coli K1 Microalgae-based wastewater treatment K1 Viral RNA reduction K1 Zero-valent iron nanoparticles K1 3108.09 Virus K1 31 Ciencias Agrarias AB Microalgae-based systems can potentially inactivate E. coli and viruses. In this work, batch algal-bacterialphotobioreactors were operated to elucidate the effect of zero-valent iron (ZVI) nanoparticles and light in-tensity on the reduction of viral RNA (MS2, Phi6 and Bovine coronavirus, BCoV) and uidA gene (E. coli) duringsecondary wastewater treatment. Biodegradation and abiotic control photoreactors were operated at high lightintensity (1100 μE m 2 s 1), with and without ZVI-nanoparticles addition (HLNP and HL) and low light intensity(450 μE m 2 s 1), without nanoparticles (LL). After 72 h, HLNP matched or increased the reductions of at least99.9 % for viruses and 99 % for uidA achieved within 192 h in HL and LL. Oxidative reactions in the presence ofZVI-nanoparticles seemed to mediate the decay of viral RNA and uidA. This work demonstrated for the first timethe potential for enhanced reduction of viral RNA and E. coli by ZVI-nanoparticles during microalgae-basedwastewater treatment. PB Elsevier SN 0960-8524 YR 2025 FD 2025 LK https://uvadoc.uva.es/handle/10324/76291 UL https://uvadoc.uva.es/handle/10324/76291 LA eng NO Bioresource Technology, 2025, vol.427, p. 132389 NO Producción Científica DS UVaDOC RD 16-jul-2025