RT info:eu-repo/semantics/article
T1 High‐Dimensional Immunophenotyping of Post‐COVID‐19 and Post‐Influenza Patients Reveals Persistent and Specific Immune Signatures After Acute Respiratory Infection
A1 Pérez‐Cózar, Francisco
A1 Cal‐Sabater, Paloma
A1 Rybakowska, Paulina
A1 Arribas‐Rodríguez, Elisa
A1 Fiz‐López, Aida
A1 García‐Blesa, Antonio
A1 Hernández, Juan
A1 Gutiérrez, Sara
A1 Tellería, Pablo
A1 Novoa, Cristina
A1 Rello, Silvia Rojo
A1 De Prado, Ángel
A1 Pérez, Cándido
A1 Sedano, Rosa
A1 Domínguez‐Gil, Marta
A1 Peñarrubia, María Jesús
A1 Pieren, Daan K. J.
A1 Garrote, José A.
A1 Arranz, Eduardo
A1 Eiros, José María
A1 Tamayo, Eduardo
A1 Orduña, Antonio
A1 van Els, Cécile A.C.M.
A1 Dueñas, Carlos
A1 Marañón, Concepción
A1 Bernardo, David
A1 Cuesta‐Sancho, Sara
K1 Inmunoterapia
K1 Covid-19 (Enfermedad)
K1 Immune signature
K1 Immunome
K1 Post‐COVID‐19
K1 Post‐influenza
K1 2412 Inmunología
K1 3202 Epidemiología
AB Long-term consequences of SARS-CoV-2 infection are unknown since recovered individuals can experience symptoms and latent viral reactivation for months. Indeed, acute post-infection sequelae have also been observed in other respiratory viral infections, including influenza. To characterize post-COVID-19 and post-influenza induced alterations to the cellular immunome, peripheral blood mononuclear cells (PBMCs) were obtained from patients 3 months after recovery from COVID-19 (n = 93) or influenza (n = 25), and from pre-pandemic healthy controls (n = 25). PBMCs were characterized using a 40-plex mass cytometry panel. Principal component analysis (PCA), classification models, and K-means clustering were subsequently applied. PCA identified distinct immune profiles between cohorts, with both post-COVID and post-flu patients displaying an altered chemokine receptor expression compared to pre-pandemic healthy controls. These alterations were more prominent in post-COVID patients since they exhibited highly increased expression of chemokine receptors CXCR3 and CCR6 by various lymphoid populations, while post-influenza patients mainly showed a decrease in CCR4 expression by naïve T cells, monocytes, and conventional dendritic cells. Classification models using immunophenotyping data confirm the three groups, while K-means clustering revealed two subgroups among post-COVID patients, with younger patients showing more pronounced immune alterations in the chemokine receptor profile, independently of long COVID symptoms. In conclusion, post-COVID and post-influenza patients exhibit distinct and unique persistent immune alterations. Understanding these altered immune profiles can guide targeted therapies for post-COVID syndrome and highlight differences in immune recovery from various respiratory infections.
PB Wiley
SN 0146-6615
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/76898
UL https://uvadoc.uva.es/handle/10324/76898
LA eng
NO Journal of medical virology, junio 2025,  97. e70435
NO Producción Científica
DS UVaDOC
RD 02-ago-2025