RT info:eu-repo/semantics/article
T1 Complex immune network and regional consistency in the human gastric mucosa revealed by high-resolution spectral cytometry
A1 De Prado, Ángel
A1 Cal-Sabater, Paloma
A1 Fiz-López, Aida
A1 Izquierdo, Sandra
A1 Corrales, Daniel
A1 Pérez-Cózar, Francisco
A1 H-Vázquez, Juan
A1 Arribas-Rodríguez, Elisa
A1 Perez-Segurado, Cándido
A1 Muñoz, Álvaro Martín
A1 Garrote, José A.
A1 Arranz, Eduardo
A1 Marañón, Concepción
A1 Cuesta-Sancho, Sara
A1 Fernández-Salazar, Luis
A1 Bernardo, David
K1 Biomedicina
K1 Inmunología
K1 Spectral cytometry
K1 Stomach
K1 Gastric
K1 H. pylori
K1 2412 Inmunología
AB The immune cellular landscape from the gastric mucosa remains largely unknown despite its relevancein several inflammatory conditions. Human gastric biopsies were obtained from the antrum, body andincisura from 10 individuals to obtain lamina propria mononuclear cells that were further characterizedby spectral cytometry. Phenotypic hierarchical analyses identified a total of 52 different immune cellsubsets within the human gastric mucosa revealing that T-cells (> 60%) and NK cells (> 20%) were themain populations. Within T-cells, CD4+ and CD8+ were equally represented with both subsets displayingmainly a memory and effector phenotype. NK cells, on the contrary, were largely of the earlyphenotype. No regional differences were observed for any subsets among the 3 locations. Followingunsupervised analysis, a total of 82 clusters were found. Again, no differences were observed amongstlocations although a great degree of inter-individual variability was found, largely influenced by thepresence of H. pylori infection and dyspepsia. We have unraveled the human gastric immune cellularsubset composition and a unique interindividual immune fingerprint with no inter-regional variations.
PB Scientific Reports
SN 2045-2322
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/76921
UL https://uvadoc.uva.es/handle/10324/76921
LA eng
NO Scientific Reports, 2024, n.14, 28685
NO Producción Científica
DS UVaDOC
RD 05-ago-2025