RT info:eu-repo/semantics/article
T1 Chitin and chitosan quantification in fungal cell wall via Raman spectroscopy
A1 Barroso Solares, Suset
A1 López Moya, Federico
A1 Fraile, Teresa
A1 Prieto Colorado, Ángel Carmelo
A1 López Llorca, Luis
A1 Pinto Sanz, Javier
K1 Biocontrol fungi
K1 Chitin
K1 Chitosan
K1 Aminopolysaccharides
K1 Raman spectroscopy
K1 31 Ciencias Agrarias
AB Investigation of cell wall composition is necessary to understand the interactions between fungi and the environment as it is the external layer exposed to stimuli and detected by other organisms. Pochonia chlamydosporia and Akanthomyces lecanii, two fungal species living in the soil and infecting nematodes and insects, exhibit endophytic interactions with various plant species. Determination of cell wall composition is essential to understand the mechanisms underlying these interactions. Therefore, in this study, for the first time, we assessed the relative amounts of chitin and chitosan in the cell walls of P. chlamydosporia (PC123) and A. lecanii (69NZ, 85SCT, 126KNY, and 447SAF) via Raman spectroscopy. The isolate with the highest chitosan percentage was 69NZ, followed by 85SCT, PC123, 447SAF, and 126KNY. Moreover, combination with conventional approaches for chitin and chitosan quantification yielded quantitative results for all cell wall components. Overall, these results highlight the mechanisms by which fungi exhibit chitosan resistance and avoid detection by the host plant during root colonization.
PB Elsevier
SN 1386-1425
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/77004
UL https://uvadoc.uva.es/handle/10324/77004
LA eng
NO Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2025, vol. 334, p. 125928
NO Producción Científica
DS UVaDOC
RD 03-ago-2025