RT dataset T1 Enrichment of mesophilic and thermophilic mixed microbial cultures for syngas biomethanation in bubble column bioreactors under continuous operation A1 Rodríguez-Gallego, Víctor A1 Bucci, Paula A1 Lebrero Fernández, Raquel A1 Muñoz Torre, Raúl A2 Rodríguez-GallegoVíctor K1 Biomethane K1 Microbial enrichment K1 Mixed microbial consortia K1 Syngas biomethanation K1 Temperature K1 2414.08 Procesos Microbianos AB The dataset compiled in this spreadsheet contains the experimental data used for the development and analysis presented in the associated research paper, regarding the evaluation of bioreactors performance and activity tests. The corpus includes measurements and observations obtained from two experimental setups aimed at investigating syngas biomethanation and the involved metabolic pathways: the continuous bioreactor experiments and batch activity tests in serum bottles. - Bioreactor Experimental Data [Inoculum, BCB1 and BCB2] This section documents the operation of two lab-scale bioreactors for syngas biomethanation under different operational conditions. The dataset includes: - Inoculum characterization - Sampling dates and experimental timepoints - Reactor operating conditions - Inlet and outlet gas compositions (H₂, CO, CO₂, CH₄, O₂, N₂) - TOC/TN evolution - TS/VS evolution - VFAs concentration measurements - pH evolution - Serum Bottle Activity Tests [M. activity tests, T. activity tests and Activity tests summary] This section contains the results of batch tests conducted in sealed serum bottles to evaluate the metabolic activity of microbial cultures under varying substrates addition. The dataset includes: - Assay type (regarding substrates employed) - Initial and final pH measurements - Headspace pressure measurements - Headspace gas measurements (CO, H₂, CO₂, CH₄, O₂, N₂) - Incubation temperture conditions - VFAs concentration measurements - Calculated product yieldsThe data provided insight into microbial activity under the addition of different substrates and were used to evaluate and identify the metabolic pathways involved in the developed syngas biomethanation process. YR 2025 FD 2025 LK https://uvadoc.uva.es/handle/10324/77355 UL https://uvadoc.uva.es/handle/10324/77355 LA eng NO Rodríguez-Gallego, V., Bucci, P., Lebrero, R., & Muñoz, R. (2025). Enrichment of mesophilic and thermophilic mixed microbial cultures for syngas biomethanation in bubble column bioreactors under continuous operation. Bioresource Technology, 437, 133082. NO Institute of Sustainable Processes (ISP) DS UVaDOC RD 23-sep-2025