RT info:eu-repo/semantics/article T1 Molecular and structural changes in starch and proteins induced by microwave treatment and their effect on pasting properties. A study on amaranth, buckwheat, quinoa, and sorghum in grain and flour form A1 Vicente Fernández, Ainhoa A1 Mauro, Raúl Ricardo A1 Villanueva Barrero, Marina A1 Caballero, Pedro A. A1 Hamaker, Bruce R. A1 Ronda Balbás, María Felicidad K1 Asymmetric flow field-flow fractionation K1 Size-exclusion chromatography K1 Confocal scanning laser microscopy K1 Pasting properties K1 Microwave treatment K1 2302 Bioquímica AB This study investigated the effects of microwave treatment (MWT) on the molecular structure and properties ofstarch and proteins, as well as on the pasting properties of flours. MWT was performed under uniform conditions(25 % moisture, 100 ◦C, 30 min) for amaranth, buckwheat, quinoa, and sorghum in two forms: grain and flour.MWT generally caused fragmentation of amylose and amylopectin, reducing amylopectin molar mass by up to39 % and amylose long chains by up to 18 %, disrupting protein secondary structure (α-helix content up to 5%), and altering morphology of starch granules and protein. All flours presented an orthorhombic crystalstructure that was maintained after MWT. MWT changes were generally more pronounced in flour than in graintreatments, with amaranth showing the greatest change and buckwheat the least. Microwave-induced changes inpasting properties depended on intrinsic flour properties and structural modifications. Principal componentanalysis revealed a consistent change in all treated samples, but to varying degrees depending on botanical originand treatment form. This study contributes to a deeper understanding of structural changes of starch and proteinsby MWT and their contribution to altered pasting properties depending on whether the treatment is applied tograin or flour. PB Elsevier SN 0141-8130 YR 2025 FD 2025 LK https://uvadoc.uva.es/handle/10324/78252 UL https://uvadoc.uva.es/handle/10324/78252 LA eng NO International Journal of Biological Macromolecules, 2025, vol. 318, p. 145094 NO Producción Científica DS UVaDOC RD 03-oct-2025