RT info:eu-repo/semantics/article
T1 Biogenic hydrogen production from household food waste via lactate-driven dark fermentation: A comparative study of single-stage and two-stage configurations
A1 Regueira Marcos, Lois
A1 Muñoz Torre, Raúl
A1 García Depraect, Octavio
K1 Dark fermentation
K1 Hydrogen production
K1 Hydraulic retention time
K1 Lactate
K1 Organic waste
K1 Waste valorization
K1 3308 Ingeniería y Tecnología del Medio Ambiente
AB Lactate-driven dark fermentation (LD-DF) is a novel approach that integrates lactate production with its con-version to biohydrogen (H2), offering a solution to the inhibition of hydrogen-producing bacteria by lactic acidbacteria while enhancing process stability. This study investigates the efficacy of LD-DF for continuous H2production by comparing the performance of a two-stage system (lactate fermentation followed by LD-DF) with asingle-stage configuration. Household food waste (FW) was used as the substrate, and the impact of varying thehydraulic retention time (HRT; 12, 8, and 6 h) was assessed in both configurations. In the two-stage system, aprogressive reduction in HRT resulted in the highest volumetric H2 production rate (HPR) of 4.4 ± 0.4 L-H2/L-d and a hydrogen yield (HY) of 22.9 ± 2.1 mL-H2/g-VSadded. In contrast, the single-stage configuration exhibiteda decline in HPR from 3.8 ± 0.6–1.4 ± 0.3 L H2/L-d when the HRT decreased from 12 h to 6 h, although itachieved the highest HY of 39.5 ± 6.0 mL-H2/g-VSadded. Principal component analysis identified a positivecorrelation between HPR and butyrate concentrations, a trend predominantly observed in the two-stageconfiguration. Conversely, the HPR negatively correlated with high levels of lactate, acetate, and propionate,which were more prevalent in the single-stage system. Interestingly, Veillonella and Bacteroides were identified asthe main H2 producers during LD-DF in both configurations. These findings demonstrate that lactic acid pre-fermentation enhances H2 productivity in FW LD-DF systems and facilitates operation at lower HRTscompared to single-stage configurations
PB Elsevier
SN 2213-3437
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/78267
UL https://uvadoc.uva.es/handle/10324/78267
LA eng
NO Journal of Environmental Chemical Engineering, 2025, vol. 13, n. 5, p. 117672
NO Producción Científica
DS UVaDOC
RD 15-nov-2025