RT info:eu-repo/semantics/article
T1 Advanced characterization in molecularly imprinted Polypyrrole for potentiometric lactose sensing
A1 Pérez González, Clara
A1 García Hernández, Celia
A1 García Cabezón, Ana Cristina
A1 Rodríguez Méndez, María Luz
A1 Martín Pedrosa, Fernando
K1 Molecularly imprinted polymers (MIPs)
K1 Polypyrrole (PPy)
K1 Potenciometric sensor
K1 Electrochemical characterization
K1 Surface characterization
K1 Lactose
K1 2302 Bioquímica
AB The motivation behind this study comes from the necessity to monitor lactose levels in milk for quality controland public health, as existing methods are often complex, time-consuming, and expensive. This research aims todevelop a specific and sensitive potentiometric sensor for lactose detection using electropolymerised polypyrrole-based molecularly imprinted polymers (MIPs). MIP sensors were developed through chronoamperometric elec-tropolymerisation of pyrrole in the presence of lactose, creating specific binding sites. Raman spectroscopy,Scanning Electron Microscopy (SEM), and Atomic Force Microscopy (AFM) were used to assess surfacemorphology confirming the presence of imprinted cavities and surface changes after lactose removal. Theelectrochemical performance of the sensors was evaluated by EIS spectroscopy demonstrating the influence ofMIP cavities on the electron transfer of the sensor compared to non-imprinted polymer (NIP). Finally, opencircuit potentials (OCP) in various lactose concentrations and real milk samples confirmed the high sensitivityand selectivity of the MIP sensors. Moreover, Principal Component Analysis (PCA), Partial Least SquareRegression (PLS), and Support Vector Machine (SVM) models were satisfactorily employed to establish corre-lations between OCP measurements and lactose content, allowing its prediction in milk samples with an averageerror of 6 %. The results demonstrated that the MIP sensors exhibited high selectivity and sensitivity towardslactose, with improved responses compared to NIP sensors. The study concludes that polypyrrole-based MIPsprovide a robust and effective approach for lactose detection and prediction in dairy products, offering apromising tool for quality control and ensuring consumer safety
PB Elsevier
SN 0026-265X
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/78654
UL https://uvadoc.uva.es/handle/10324/78654
LA eng
NO Microchemical Journal, 2025, vol. 216, p. 114709
NO Producción Científica
DS UVaDOC
RD 18-oct-2025