RT info:eu-repo/semantics/article T1 Towards syngas biorefineries: The potential of microbial consortia for syngas valorisation A1 García Casado, Silvia María A1 Muñoz Torre, Raúl A1 Lebrero Fernández, Raquel K1 Alcohols K1 Carboxylic acids K1 Gasification K1 Mass transfer K1 Methane K1 Mixed cultures K1 Syngas K1 3308 Ingeniería y Tecnología del Medio Ambiente AB Gasification has emerged as a promising platform to cope with recalcitrant organic waste within the frameworkof biomass-based biorefineries, producing syngas that can be fermented into valuable bioproducts. Despite itspotential, syngas fermentation is based predominantly on pure cultures, which faces significant challenges,including the limited portfolio of generated compounds (primarily acetate and ethanol) and their low produc-tivity. To address these bottlenecks, the potential of microbial consortia as effective platforms for syngas con-version has been evaluated. Syngas biomethanation using mixed cultures is a well-established process, with pilot-scale implementations yielding promising results. Alternatively, the production of carboxylic acids has emergedas an interesting option compared to pure cultures, as comparable acetate productivities can be achieved alongwith the possibility for chain elongation to butyrate or caproate. However, the feasibility of using mixed culturesto produce alcohols and other high-value compounds from syngas remains underexplored. Advancing the fieldwill also require the development of innovative technologies to overcome inherent barriers and fully unlock thepotential of syngas-based bioprocesses. Key challenges include the presence of impurities and variability insyngas composition, mass transfer limitations in bioreactors, and the need for efficient downstream effluentpurification. In this context, mixed cultures emerge as a robust approach capable of buffering syngas fluctuationsand tolerating certain impurities. At the same time, the development of novel gas phase bioreactors and inno-vative membrane-based systems for effluent purification is crucial for enhancing CO and H2 mass transfer andimproving products titers, respectively. PB Elsevier SN 0734-9750 YR 2025 FD 2025 LK https://uvadoc.uva.es/handle/10324/78732 UL https://uvadoc.uva.es/handle/10324/78732 LA eng NO Biotechnology Advances, 2025, vol. 85, p. 108699 NO Producción Científica DS UVaDOC RD 13-nov-2025