RT info:eu-repo/semantics/article
T1 Occurrence and biological activity of palmitoleic acid isomers in phagocytic cells
A1 Astudillo, Alma M.
A1 Meana González, Clara
A1 Guijas Maté, Carlos
A1 Pereira, Laura
A1 Lebrero Fernández, Patricia
A1 Balboa, María A.
A1 Balsinde Rodríguez, Jesús
K1 Bioquímica
K1 Inmunología
K1 Biología celular
K1 Metabolismo
K1 mediadores lipídicos
K1 monocitos/macrófagos
K1 inflamación
K1 2302 Bioquímica
K1 2412 Inmunología
K1 2407 Biología Celular
AB Recent studies have highlighted the role of palmitoleic acid [16:1n-7 (cis-9-hexadecenoic acid)] as a lipid hormone that coordinates cross-talk between liver and adipose tissue and exerts anti-inflammatory protective effects on hepatic steatosis and insulin signaling in murine models of metabolic disease. More recently, a 16:1n-7 isomer, cis-7-hexadecenoic acid (16:1n-9), that also possesses marked anti-inflammatory effects, has been described in human circulating monocytes and monocyte-derived macrophages. By using gas chromatographic/mass spectrometric analyses of dimethyl disulfide derivatives of fatty acyl methyl esters, we describe in this study the presence of a third 16:1 isomer, sapienic acid [16:1n-10 (6-cis-hexadecenoic acid)], in phagocytic cells. Cellular levels of 16:1n-10 appear to depend not only on the cellular content of linoleic acid, but also on the expression level of fatty acid desaturase 2, thus revealing a complex regulation both at the enzyme level, via fatty acid substrate competition, and directly at the gene level. However, unlike 16:1n-7 and 16:1n-9, 16:1n-10 levels are not regulated by the activation state of the cell. Moreover, while 16:1n-7 and 16:1n-9 manifest strong anti-inflammatory activity when added to the cells at low concentrations (10 μM), notably higher concentrations of 16:1n-10 are required to observe a comparable effect. Collectively, these results suggest the presence in phagocytic cells of an unexpected variety of 16:1 isomers, which can be distinguished on the basis of their biological activity and cellular regulation.
PB Elsevier
SN 0022-2275
YR 2018
FD 2018
LK https://uvadoc.uva.es/handle/10324/79493
UL https://uvadoc.uva.es/handle/10324/79493
LA eng
NO Journal of Lipid Research, Feb 2018, vol. 59, n. 2, p. 237-249
NO Producción Científica
DS UVaDOC
RD 05-mar-2026