RT info:eu-repo/semantics/article T1 Hydrothermal pretreatment of brewer's spent grain: A pathway to sustainable biogas production and waste valorization A1 Bucci, Paula A1 Cantero Sposetti, Danilo Alberto A1 Casas González, Andrea Patricia A1 Marcos Naveira, José Enrique A1 Menalla, Enkeledo A1 Muñoz Torre, Raúl K1 Brewer's spent grain K1 Continuous anaerobic digestion K1 Hydrothermal pretreatment K1 Microbial community dynamics K1 Methanogenic performance K1 Renewable energy recovery K1 23 Química AB Brewer’s spent grain (BSG), a by-product of the brewing industry, faces significant waste management challengesbut holds potential as a substrate for biogas production via anaerobic digestion (AD). However, its lignocellulosiccomplexity limits microbial degradation and methane yield. In previous studies by our research group, varioustreatment strategies for BSG were explored, including approaches aimed at recovering high-value compounds.Building upon this foundation, the present work evaluates the potential of hydrothermal pretreatment to enhancemethane production from BSG via AD. The novelty lies in the combination of a continuous hydrothermal pre-treatment reactor with meta-transcriptomic analysis of the anaerobic digestion process, enabling a direct cor-relation between operational performance and microbial functional shifts. Hydrothermal pretreatment wasapplied to partially hydrolyze cellulose and hemicellulose into fermentable sugars, improving biodegradabilitywhile minimizing the formation of inhibitory compounds. Results showed that hydrothermally pretreated BSGsupported methane productions over 1500 mL CH4/L_reactor⋅day, markedly higher than the 100–500 mL CH4/L_reactor⋅day observed for untreated BSG. Volatile solids degradation efficiency improved by 30 %, whilemethane content in biogas increased from 30 % to 65 %. Genomic analysis of the microbial consortium revealedenhanced activity of methanogenic archaea and fermentative bacteria associated with the increased methaneproduction. This integrated approach not only disrupts lignocellulosic barriers more effectively but also providesdeeper insights into microbial functionality, reinforcing hydrothermal pretreatment as a viable strategy forboosting biogas yield and advancing sustainable waste-to-energy solutions. PB Elsevier SN 0961-9534 YR 2026 FD 2026 LK https://uvadoc.uva.es/handle/10324/79510 UL https://uvadoc.uva.es/handle/10324/79510 LA eng NO Biomass and Bioenergy, 2025, vol. 204, p. 108399 NO Producción Científica DS UVaDOC RD 10-nov-2025