RT info:eu-repo/semantics/article
T1 Bioorthogonal mussel-inspired elastin-like nanocoatings for indwelling devices
A1 Acosta Rodríguez, Sergio
A1 Chaskovska, Viktoriya
A1 El-Maachi, Ikram
A1 Englert, Jenny
A1 Puertas Bartolomé, María
A1 Jockenhoevel, Stefan
A1 Rodríguez Cabello, José Carlos
A1 Rodríguez Emmenegger, César
A1 Fernández Colino, Alicia
K1 Elastin-like recombinamers
K1 Biofunctional coatings
K1 Surface functionalization
K1 DOPA
K1 Click chemistry
K1 2302 Bioquímica
AB Medical devices such as vascular grafts, stents, and catheters are crucial for patient treatment but often suffer suboptimal integration with host tissues due to the nature of their surfaces. The materials commonly used, including metals and synthetic polymers, frequently lead to undesired immune responses and device failure. In this context, coating their surfaces with designer proteins has arisen as a promising strategy to improve the device’s biointegration. Here, we present a bioinspired method for coating biomaterial surfaces with protein-engineered polymers designed to mimic tailored functions from the native extracellular matrix (ECM). Combining mussel-inspired catechol chemistry with bioorthogonal click chemistry, we developed a modular grafting method for the surface functionalization of metallic and polymeric implants using a bifunctional peptide containing azide and DOPA (3,4-dihydroxyphenylalanine) groups. This simple dip-coating process enabled the fabrication of bioactive elastin-like coatings with precise peptide presentation. The results reveal enhanced bioactivity and cytocompatibility, as evidenced by improved endothelial cell adhesion, proliferation, and heparin-binding capacity on coated surfaces. The versatility and effectiveness of this bioorthogonal coating method suggest significant potential for creating implant surfaces tailored to diverse clinical applications.
PB American Chemical Society
SN 1944-8244
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/79561
UL https://uvadoc.uva.es/handle/10324/79561
LA eng
NO ACS Applied Materials & Interfaces, 2025, vol. 17, n. 36, p. 50279-50291
NO Producción Científica
DS UVaDOC
RD 06-dic-2025