RT info:eu-repo/semantics/article
T1 Enrichment of mesophilic and thermophilic mixed microbial cultures for syngas biomethanation in bubble column bioreactors under continuous operation
A1 Rodríguez Gallego, Víctor
A1 Bucci, Paula
A1 Lebrero Fernández, Raquel
A1 Muñoz Torre, Raúl
K1 Biomethane
K1 Biomethane
K1 Mixed microbial consortia
K1 Syngas biomethanation
K1 Temperature
K1 2414.08 Procesos Microbianos
AB Syngas biomethanation—microbial conversion of CO2, CO, and H2 into CH4—offers a promising strategy forupcycling gas streams from organic waste gasification. This study investigated the effect of temperature onmicrobial community enrichment and performance during syngas conversion in continuous bubble columnbioreactors operated under mesophilic (30 ◦ C) and thermophilic (50 ◦C) conditions at increasing H2 loads. At thehighest H2 load, the CH4 content in the biogas produced was approximately 84.6 ± 1.4 % under mesophilicconditions and 87.3 ± 1.3 % under thermophilic conditions. The study of the metabolic pathways revealed thedominance of syntrophic acetate oxidation, hydrogenotrophic methanogenesis, homoacetogenesis, and carbox-ydotrophic acetogenesis in the mesophilic consortium, while hydrogenotrophic methanogenesis and carbox-ydotrophic hydrogenogenesis dominated in the thermophilic consortium. Similarly, 16S rRNA metataxonomicanalysis revealed shifts in microbial diversity, with Methanobacterium, Acetobacterium, and Anaerolineaceaedominating the mesophilic culture, and Methanothermobacter and carboxydotrophic Firmicutes accounting foralmost the entire thermophilic community. These findings provide guidance for selecting mesophilic or ther-mophilic conditions based on preferred metabolic routes, microbial community composition, and process goals.
PB Elsevier
SN 0960-8524
YR 2025
FD 2025
LK https://uvadoc.uva.es/handle/10324/80082
UL https://uvadoc.uva.es/handle/10324/80082
LA eng
NO Bioresource Technology, 2025, vol. 437, p. 133082
NO Producción Científica
DS UVaDOC
RD 26-nov-2025