RT info:eu-repo/semantics/article
T1 Deep eutectic solvent-based protein recovery from microalgal biomass grown in piggery wastewater
A1 Moldes Plaza, David
A1 Fernández Requejo, Patricia
A1 Vega Alegre, María del Sol
A1 Bolado Rodríguez, Silvia
K1 Biodegradable solvents
K1 Microalgae Biorefinery
K1 Scenedesmus almeriensis
K1 Solid-liquid extraction
K1 Swine wastewater
K1 Taguchi design of experiments
K1 3308 Ingeniería y Tecnología del Medio Ambiente
AB Microalgae are a promising alternative to address wastewater treatment while converting wastewater nutrients into valuable protein-rich biomass, providing a dual-purpose solution. This study investigates protein recovery from microalgal biomass based on Scenedesmus almeriensis cultivated in swine wastewater using deep eutectic solvents (DESs) and their water mixtures. The behavior of two DESs (choline chloride:urea and choline chloride:glycerol at a 1:2 molar ratio) was evaluated with varying amounts of water. Both DESs outperformed water in protein extraction, and adding water to the DES:biomass mixture improved recoveries. The impact of the experimental factors was evaluated using a factorial design. The highest protein yield (16.8 %) was obtained with DES ChCl:2Urea with 81.6 % of water, by milling discs, a 12:1 DES:biomass ratio and extraction at 30 °C by 0.5 h. Despite moderate yields, this work underscores the potential of aqueous solutions of DESs as green solvents for protein recovery from microalgae.
PB Elsevier
SN 0960-8524
YR 2026
FD 2026
LK https://uvadoc.uva.es/handle/10324/80083
UL https://uvadoc.uva.es/handle/10324/80083
LA eng
NO Bioresource Technology, 2026, vol. 440, p. 133446
NO Producción Científica
DS UVaDOC
RD 26-nov-2025