RT info:eu-repo/semantics/article
T1 An Evolutionary Perspective of the Lipocalin Protein Family
A1 Diez-Hermano, Sergio
A1 Ganfornina Álvarez, María Dolores
A1 Skerra, Arne
A1 Gutiérrez, Gabriel
A1 Sánchez Romero, Diego
AB The protein family of Lipocalins is ubiquitously present throughout the tree of life, withthe exception of the phylum Archaea. Phylogenetic relationships of chordate Lipocalinshave been proposed in the past based on protein sequence similarities, but theirhighly divergent primary structures and a shortage of experimental annotations ingenome projects have precluded a well-supported hypothesis for their evolution. In thiswork we propose a novel topology for the phylogenetic tree of chordate Lipocalins,inferred from multiple amino acid sequence alignments. Sixteen jawed vertebrates withfair coverage by genomic sequencing were compared. The selected species spanan evolutionary range of  400 million years, allowing for a balanced representationof all major vertebrate clades. A consensus phylogenetic tree is proposed followinga comparison of sequence-based maximum-likelihood trees and protein structuredendrograms. This new phylogeny suggests an APOD-like common ancestor in earlychordates, which gave rise, via whole-genome or tandem duplications, to the sixLipocalins currently present in fish (APOD, RBP4, PTGDS, AMBP, C8G, and APOM).Further gene duplications of APOM and PTGDS resulted in the altogether 15 Lipocalinsfound in contemporary mammals. Insights into the functional impact of relevant aminoacid residues in early diverging Lipocalins are also discussed. These results shouldfoster the experimental exploration of novel functions alongside the identification of newmembers of the Lipocalin family.
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/80515
UL https://uvadoc.uva.es/handle/10324/80515
LA spa
NO Front Physiol. 2021 Aug 23;12:718983
NO Producción Científica
DS UVaDOC
RD 12-feb-2026