RT info:eu-repo/semantics/article
T1 Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency
A1 Galindo, Sara
A1 López Paniagua, Marina
A1 De La Mata Sampedro, Ana
A1 Herreras Cantalapiedra, José María
A1 García Vázquez, Carmen
A1 Marceñido, Beatriz
A1 Rey, Esther
A1 Higuera Barón, Celia
A1 Calonge, Margarita
A1 Nieto Miguel, Teresa
K1 Cornea
K1 Corneal epithelium
K1 Limbus
K1 Limbal stem cell deficiency
K1 Mesenchymal stem cells
K1 Regeneration
K1 Stem cell transplantation
K1 3201.09 Oftalmología
AB Ocular limbal stem cell deficiency (LSCD) occurs because of corneal epithelial stem cell destruction ordysfunction at the limbal niche. LSCD can cause corneal blindness, and the current therapy based on limbal stemcell transplantation is continuously improving. The aim of this work was to compare the safety and efficacy ofhuman mesenchymal stem cells (hMSCs) derived from bone marrow (hBM-MSCs) and adipose tissue (hAT-MSCs)when transplanted to a rabbit model of LSCD. Both hMSC types expressed the corneal and limbal epithelial cellmarkers CK3, CK12, ZO-1, and ABCG2 under standard culture conditions. A few hBM-MSCs expressed CK7 and E-cadherin, while hAT-MSCs expressed more CK7 but no E-cadherin. The hMSCs were seeded onto amnioticmembranes and transplanted onto the ocular surface of a LSCD rabbit model. Both hMSC types were welltolerated without immunosuppression and were primarily located in the superior limbal stroma eight weeks post-transplantation. The hBM-MSC–treated group showed less superficial neovascularization, while the hAT-MSC–treated group showed less conjunctival invasion and fewer corneal stromal blood vessels. Compared to theuntreated LSCD group, both hMSC-treated groups had less corneal opacity, less corneal and limbal stromalinflammation, and more corneal epithelial layers that partially recovered the corneal and limbal epithelialmarkers CK3, CK15, and p63. Overall, transplantation of hBM-MSCs and hAT-MSCs in a rabbit LSCD modelreduced the development of corneal opacity, neovascularization, inflammation, and partially restored cornealand limbal tissue structure and epithelial cell phenotypes. Therefore, both types of hMSCs could become validalternatives for LSCD treatment.
PB Elsevier
SN 0014-4835
YR 2026
FD 2026
LK https://uvadoc.uva.es/handle/10324/80765
UL https://uvadoc.uva.es/handle/10324/80765
LA eng
NO Experimental Eye Research, 2026, vol. 262, p. 110737
NO Producción Científica
DS UVaDOC
RD 18-dic-2025