RT info:eu-repo/semantics/article T1 Influence of operational parameters on the performance of domestic wastewater treatment in a single-stage algal-bacterial column photobioreactor A1 Dos Santos, Thalita Lacerda A1 Vargas Estrada, Laura Gabriela A1 Blanco, Saul A1 Da Silva, Gustavo Henrique Ribeiro A1 Muñoz Torre, Raúl K1 Biomass productivity K1 Hydraulic retention time K1 Light availability K1 Microalgal-bacterial symbiosis K1 Solid retention time K1 3308 Ingeniería y Tecnología del Medio Ambiente AB This study evaluated the performance of a single-stage photobioreactor (PBR), under varying hydraulic retention times (HRT) (4, 3 and 2 days), biomass concentrations (2.7 and 1.6 gTSS L−1) and illuminated surface/volume ratios (17.8 and 26.7 m2 m−3) in a continuous system operated for 192 days. The system achieved removal efficiencies of Total Organic Carbon of 94–96 %, Total Nitrogen of 92–97 % and phosphate of 91–100 % regardless of the operational conditions. N-NH4+ removal was primarily driven by assimilation into algal-bacterial biomass. Nitrate and nitrite accumulation in the cultivation broth was not observed and N2O generation was negligible. Increased light availability boosted biomass productivity up to 335.7 ± 77.8 g m−3 d−1. The reduction in biomass concentrations from 2.7 to 1.6 gTSS L−1 decreased microalgal concentration and diversity, particularly affecting filamentous bacteria. These results demonstrate that a single-stage PBR can achieve performance comparable to conventional two stages anoxic-aerobic photobioreactors, while being more cost-effective and sustainable, highlighting its potential for advanced wastewater treatment and resource recovery. PB Elsevier SN 2211-9264 YR 2025 FD 2025 LK https://uvadoc.uva.es/handle/10324/81019 UL https://uvadoc.uva.es/handle/10324/81019 LA eng NO Algal Research, 2025,vol. 92, p. 104417 NO Producción Científica DS UVaDOC RD 23-dic-2025