RT info:eu-repo/semantics/article T1 Valorization of polyester wastes into polyhydroxyalkanoates via a one-step microbial fermentation process A1 Santos Beneit, Fernando A1 Bordel Velasco, Sergio A1 Martín González, Diego A1 Fuente Tagarro, Carlos de la A1 García Depraect, Octavio A1 Börner, Tim A1 Börner, Rosa Aragão A1 Muñoz Torre, Raúl K1 Polyhydroxyalkanoates (PHA) K1 Poly(3-hydroxybutyrate) (PHB) K1 Paracoccus denitrificans K1 Polyesters K1 Monomers K1 Plastic bio-upcycling K1 Plastic waste K1 3308 Ingeniería y Tecnología del Medio Ambiente AB Polyhydroxyalkanoates (PHAs) are promising biodegradable alternatives to petroleum-based plastics, yet theirlarge-scale application remains hindered by high production costs. Here, a direct and cost-effective microbialstrategy to upcycle polyester waste into PHAs using Paracoccus denitrificans was investigated. Remarkably, thebacterium metabolized 10 out of 12 tested plastic-derived monomers and efficiently accumulated intracellularPHAs, even when fed with pretreated mixed polyester waste instead of purified substrates. More than 80 % ofpolyester monomers supported growth, and medium optimization through nitrogen reduction boosted PHAcontent up to 30 % of cell dry weight. This one-step process circumvents costly separation steps, enabling thevalorization of heterogeneous plastic mixtures and significantly reducing both economic and processing burdens.By transforming post-consumer plastics into high-value biopolymers, this work positions P. denitrificans as aversatile platform for circular bioeconomy strategies, directly supporting global sustainability agendas andadvancing sustainable bioprocessing for the green industry PB Elsevier SN 1359-5113 YR 2026 FD 2026 LK https://uvadoc.uva.es/handle/10324/81658 UL https://uvadoc.uva.es/handle/10324/81658 LA eng NO Process Biochemistry, 2026, vol. 161, p. 283-289 NO Producción Científica DS UVaDOC RD 21-ene-2026