RT info:eu-repo/semantics/article
T1 Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
A1 Torres Franco, Andrés Felipe
A1 Herrero Lobo, Raquel
A1 Lebrero Fernández, Raquel
A1 Muñoz Torre, Raúl
K1 Ectoine biosynthesis
K1 Ectoine extraction
K1 Methane bioconversion
K1 Taylor Flow reactor
K1 3308 Ingeniería y Tecnología del Medio Ambiente
AB Ectoine is one of the most profitable value chains for biogas valorisation. This study assessed the long-termeffects of biomilking for ectoine (Ect) and hydroxyectoine (Hy) extraction on upstream methane bioconversioninto ectoines using a halotolerant methanotrophic consortium cultivated in a Taylor-Flow bioreactor. After acontrol stage (S-I), fractions of the culture broth volume of 10 %, 50 %, 30 % and 60 % (S-II to S-VI) weresubjected to biomilking before a final control (S-VII). No adverse effects were observed at 10 %, while higherfractions led to salt depletion, a ~10 % reduction in CH4 bioconversion efficiency, and a loss of dominant ectoineproducers, mainly Methylomicrobium. A decrease in intracellular Ect was also observed. Restoring salt levels (S-VI) recovered Methylomicrobium dominance and Ect content in the culture broth. Enhanced biomilking yielded upto 27.2 g-Ect + Hy per inlet kg of CH4, supporting its feasibility for sustainable biogas valorisation at a com-mercial scale.
PB Elsevier
SN 0960-8524
YR 2026
FD 2026
LK https://uvadoc.uva.es/handle/10324/81668
UL https://uvadoc.uva.es/handle/10324/81668
LA eng
NO Bioresource Technology, 2026, vol. 444, p. 133920
NO Producción Científica
DS UVaDOC
RD 16-ene-2026