RT info:eu-repo/semantics/article
T1 Current approaches to develop “off-the-shelf” chimeric antigen receptor (CAR)-T cells for cancer treatment: a systematic review
A1 Aparicio, Cristina
A1 Acebal, Carlos
A1 González-Vallinas, Margarita
K1 Chimeric antigen receptor (CAR)-T cells
K1 Cancer immunotherapy
K1 “Off-the-shelf” adoptive T cell therapy
K1 Allogeneic treatment
K1 Advanced therapy medicinal products (ATMPs)
K1 Genetic engineering
K1 Graft-versus-host disease (GvHD)
K1 Allorejection
K1 Systematic review
AB Chimeric antigen receptor (CAR)-T cell therapy is one of the most promising advances in cancer treatment. It is based on genetically modified T cells to express a CAR, which enables the recognition of the specific tumour antigen of interest. To date, CAR-T cell therapies approved for commercialisation are designed to treat haematological malignancies, showing impressive clinical efficacy in patients with relapsed or refractory advancedstage tumours. However, since they all use the patient´s own T cells as starting material (i.e. autologous use), they have important limitations, including manufacturing delays, high production costs, difficulties in standardising the preparation process, and production failures due to patient T cell dysfunction. Therefore, many efforts are currently being devoted to contribute to the development of safe and effective therapies for allogeneic use, which should be designed to overcome the most important risks they entail: immune rejection and graft-versus-host disease (GvHD). This systematic review brings together the wide range of different approaches that have been studied to achieve the production of allogeneic CAR-T cell therapies and discuss the advantages and disadvantages of every strategy. The methods were classified in two major categories: those involving extra genetic modifications, in addition to CAR integration, and those relying on the selection of alternative cell sources/subpopulations for allogeneic CAR-T cell production (i.e. γδ T cells, induced pluripotent stem cells (iPSCs), umbilical cord blood T cells, memory T cells subpopulations, virus-specific T cells and cytokine-induced killer cells). We have observed that, although genetic modification of T cells is the most widely used approach, new approaches combining both methods have emerged. However, more preclinical and clinical research is needed to determine the most appropriate strategy to bring this promising antitumour therapy to the clinical setting.
PB BioMed Central (BMC)
SN 2162-3619
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/81894
UL https://uvadoc.uva.es/handle/10324/81894
LA eng
NO Experimental Hematology & Oncology, Agosto 2023, vol. 12, no 1, p. 73.
NO Producción Científica
DS UVaDOC
RD 20-ene-2026