RT info:eu-repo/semantics/article
T1 Pilot-scale biorefinery for the production of purified biopolymers based on hydrothermal treatment in flow-through reactor cycles
A1 Ramos Andrés, Marta
A1 Díaz Cesteros, Sergio
A1 Majithia, Natasha
A1 García Serna, Juan
K1 Autohidrólisis, films, zanahoria descartada, purificación, hemicelulosa, pectina
AB Purified solid fractions of hemicellulose and pectin biopolymers with different molecular weight were produced on a pilot-scale from discarded carrots. The pulp was subjected to hydrothermal extraction on a pilot plant operating in cycles of three flow-through reactors in series at 140 and 180 °C. Two operational modes of start-up (with and without water pre-filling) were tested, showing that it influences the final hydrolysate concentration and the stability of the system. Free sugars, arabinogalactan hemicellulose and homogalacturonan pectin were produced at maximum recoveries of 379.5 g/kg dry pulp, 81.0 g/kg dry pulp and 5.35 g/kg dry pulp, respectively, with the extraction of arabinogalactan reaching a yield of 96.1 % (w/w). The extracted biopolymers were separated and purified through ultrafiltration and diafiltration cycles using a multi-membrane system (30, 10, 1 kDa). Ultrafiltration and especially diafiltration allowed going from extracts with molecular weight, polydispersity and purity values of 14.77 kDa, 19.2 and 22.2 % w/w (140 °C extract) and 8.08 kDa, 18.2 and 14.9 % w/w (180 °C extract), to fractions with values of 80.36 kDa, 67.77 kDa, 9.85 kDa, 5.23 kDa and 3.86 kDa (molecular weight), 1.3 – 3.8 (polydispersity), and 64.5 – 100 % w/w (purity). The five fractions were freeze-dried and spray-dried. The pilot-scale system allowed the production of purified biopolymer fractions of high purity, different average molecular weight, and in sufficient quantity for their subsequent transformation into products such as biodegradable films.
SN 1385-8947
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/82396
UL https://uvadoc.uva.es/handle/10324/82396
LA eng
NO Chemical Engineering Journal, Volume 437, Part 1, 1 June 2022, 135123
NO Producción Científica
DS UVaDOC
RD 17-mar-2026