RT info:eu-repo/semantics/article
T1 Mild One-Step Protein Recovery from Microalgae Cultivated in Swine Wastewater Using Natural Deep Eutectic Solvent-Based Aqueous Biphasic Systems
A1 Moldes Plaza, David
A1 Vega Alegre, María del Sol
A1 Bolado Rodríguez, Silvia
A1 Fernández Requejo, Patricia
K1 Separation (Technology)
K1 Protein extraction
K1 Green solvents
K1 Biomass valorization
K1 Microalgae biomass
K1 Aqueous two-phase system (ATPS), Natural deep eutectic solvents (NADES), Protein recovery, Green extraction, Microalgae biomass, Biomass Valorization
K1 3303.04 Separación Química
K1 2301.02 Análisis Bioquímico
AB Photobioreactor-based microalgae cultivation offers an integrated approach for nutrient-rich wastewater treatment while producing valuable biomass. One of the main microalgae components is proteins, making them a biotechnological target. In this work, to develop efficient and greener extraction methodologies, aqueous two-phase systems (ATPSs) based on natural deep eutectic solvents (NADESs) were evaluated for one-step protein extraction from microalgae cultivated in swine wastewater. Six ATPSs combining two NADES—betaine:levulinic acid (Bet:2LA) and choline chloride:urea (ChCl:2Urea)—and their individual components (Bet or ChCl) with phosphate salts were compared. Systems {NADES + K3PO4 + water} were characterized and reported for the first time. Protein recovery yield (PRY) and selectivity (protein-to-carbohydrate mass ratio, R) were assessed for three extraction times and at room temperature. The ATPS {Bet:2LA + K3PO4 + H2O} achieved a PRY of 16.4% and remarkable selectivity after 30 min (R = 2.17 g/g), with proteins concentrated in the NADES-rich phase, and negligible recovery in the salt-rich phase. Although the maximum PRY (18.2% at 120 min) was achieved with the precursor betaine, the ATPS with Bet:2LA at 30 min offered an optimal balance between efficiency and process time. With a water content of up to 50%, these systems underscore the potential of NADES-based ATPSs as sustainable platforms forprotein recovery.
PB MDPI
SN 1420-3049
YR 2026
FD 2026
LK https://uvadoc.uva.es/handle/10324/82404
UL https://uvadoc.uva.es/handle/10324/82404
LA eng
NO Molecules, 2026, 31, 483
NO Producción Científica
DS UVaDOC
RD 30-ene-2026