RT info:eu-repo/semantics/article
T1 Protein extraction from seaweed Saccharina latissima with deep eutectic solvents
A1 Moldes, David
A1 Requejo, Patricia F.
A1 Vega, Marisol
A1 Bolado, Silvia
A1 Wijffels, René H.
A1 Kazbar, Antoinette
K1 Separation (Technology)
K1 Protein extraction
K1 Macroalgae biomass
K1 Green solvents
K1 Biomass valorization
K1 Aqueous two-phase system (ATPS), Natural deep eutectic solvents (NADES), Protein recovery, Green extraction, Macroalgae biomass, Biomass Valorization
K1 3303.04 Separación Química
K1 3303.84 Biorrefinerías
K1 2301.02 Análisis Bioquímico
AB Interest in seaweed as a sustainable source of protein is growing, and deep eutectic solvents (DESs) are apromising green alternative with proven efficacy in protein extraction. This work studies the selective extraction of protein towards carbohydrates from the brown algae Saccharina latissima using DESs. Eleven DESs based on choline chloride (ChCl) and betaine were tested for freeze-dried biomass. The four DESs with the best performance (ChCl:Oxalic acid, ChCl:2Urea, ChCl:2Levulinic acid and etaine:2Urea:Water) were subsequently used to investigate the effect of temperature (20, 30 and 40 ºC) and the addition of water (0 and 47 %) on the extraction process. Betaine:2Urea:Water at 1 h, 40 ºC, and 47 % added water provided the highest protein recovery yield (10.6 %) while minimizing carbohydrate extraction (1.3 %). These results demonstrated similar protein recovery yields to a benchmark process using bead milling, but with better selectivity. Thus, this work provides a sustainable extraction method for the recovery of proteins from seaweed and open new alternatives combining both approaches.
PB Elsevier
SN 0026-265X
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/82405
UL https://uvadoc.uva.es/handle/10324/82405
LA eng
NO Microchemical Journal, 2024, 205, 111275
NO Producción Científica
DS UVaDOC
RD 30-ene-2026