RT info:eu-repo/semantics/article
T1 Separation and characterization of the structural features of macromolecular carbohydrates from wild edible mushrooms
A1 Villares, Ana
A1 García-Lafuente, Ana
A1 Guillamón, Eva
A1 Mateo-Vivaracho, Laura
K1 Cantharellus cibarius
K1 Polysaccharide
K1 Congo red
K1 NMR
K1 Chanterelle
K1 Mushroom
AB A structural characterization of two polysaccharides isolated from the fruiting bodies of the wild edible mushroom Cantharellus cibarius was performed after extraction and purification. Two polysaccharides were separated by successive aqueous extractions with boiling water and with NaOH aqueous solution (1 M, 100°C), respectively. The size exclusion chromatograms from the separated fractions showed a single peak in each fraction. The polysaccharide from the boiling water fraction (PsCcib-I) was a glucan-type carbohydrate with a molecular weight of 150 kDa. The methylation analysis and NMR experiments showed that PsCcib-I was composed of a main chain consisting of α-(1→6)-Glcp units with β-(1→4)-linked branches every third glucose residue. Differently, the polysaccharide separated from the hot aqueous NaOH fraction (PsCcib-II) had a molecular weight of 120 kDa and appeared to be a glucan-type carbohydrate composed of β-(1→3)-linked glucose units with branches at O-6 every third sugar residue. The complexation with Congo red showed that PsCcib-II displayed a triple helical conformation. © 2013 Elsevier Ltd. All rights reserved.
PB Elsevier Ltd.
SN 2212-6198
YR 2013
FD 2013
LK https://uvadoc.uva.es/handle/10324/82436
UL https://uvadoc.uva.es/handle/10324/82436
LA spa
NO Bioactive Carbohydrates and Dietary Fibre 2 (2013) 15–21
DS UVaDOC
RD 02-feb-2026