RT info:eu-repo/semantics/article T1 A green strategy to transform pineapple peel into biobutanol includes microwave pretreatment and in-situ butanol recovery. A1 Moraes Altafini, Rafael de A1 López Linares, Juan Carlos A1 González-Galán, Alba Mei A1 García Cubero, María Teresa A1 Reginatto, Valeria A1 Coca Sanz, Mónica K1 Clostridium beijerinckii K1 gas stripping K1 saccharification K1 fruit waste K1 thermal pretreatment K1 3302 Tecnología Bioquímica K1 3303 Ingeniería y Tecnología Químicas K1 2303 Química Inorgánica AB Pineapple peel, a residue rich in free sugars, can be hydrolyzed under mild conditions. Here, direct enzymatic hydrolysis (EH) of pineapple peel in the presence of Cellic CTec 2 at only 5 FPU g-¹ of pineapple peel dry mass yielded a hydrolysate containing 50.3 g L-¹ total sugars, but which was poorly fermentable. Pretreating the pineapple peel withmicrowave (MW) at 130 °C in the presence of water provided a fermentable residue containing 43.7 g L-¹ total sugars that generated 8.4 g L-¹ butanol. Thus, thermal treatment helped to overcome fermentation hindrance. Treating the pineapple peel with MW at 130 °C followed by EH at enzyme loading of 5 FPU g-1 DM gave a fermentable hydrolysate containing 50.6 g L-¹ total sugars. Fermenting this hydrolysate in a bioreactor with in-situ butanol recovery by gas stripping with a pulse of glucose/fructose solution increased the butanol concentration to 9.8 ± 0.4 g L-¹. Overall, microwave pretreatment was essential to obtain a fermentable hydrolysate from pineapple peel, while gas stripping facilitated butanol removal during fermentation. PB Elsevier Ltd. YR 2026 FD 2026 LK https://uvadoc.uva.es/handle/10324/82464 UL https://uvadoc.uva.es/handle/10324/82464 LA eng NO de Moraes Altafini R, López-Linares JC, González-Galán AM, Garcia- Cubero MT, Reginatto V, Coca M, A green strategy to transform pineapple peel into biobutanol includes microwave pretreatment and in-situ butanol recovery., Renewable Energy, 125341. https://doi.org/10.1016/ j.renene.2026.125341. NO Producción Científica DS UVaDOC RD 03-feb-2026