RT info:eu-repo/semantics/article
T1 Cellular uptake of multilayered capsules produced with natural and genetically engineered biomimetic macromolecules
A1 Costa, Rui R.
A1 Girotti, Alessandra
A1 Santos, Mercedes
A1 Arias, F. Javier
A1 Mano, João F.
A1 Rodríguez-Cabello, J. Carlos
AB Multilayered microcapsules of chitosan and biomimetic elastin-like recombinamers (ELRs) were prepared envisaging the intracellular delivery of active agents. Two ELRs containing either a bioactive RGD sequence or a scrambled non-functional RDG were used to construct two types of functionalized polymeric microcapsules, both of spherical shape ∼4 μm in diameter. Cell viability studies with human mesenchymal stem cells (hMSCs) were performed using microcapsule/cell ratios between 5:1 and 100:1. After 3 and 72 h of co-incubation, no signs of cytotoxicity were found, but cells incubated with RGD-functionalized microcapsules exhibited higher viability values than RDG cells. The internalization efficacy and bioavailability of encapsulated DQ-ovalbumin were assessed by monitoring the fluorescence changes in the cargo. The data show that surface functionalization did not significantly influence internalization by hMSCs, but the bioavailability of DQ-ovalbumin degraded faster when encapsulated within RGD-functionalized microcapsules. The microcapsules developed show promise for intracellular drug delivery with increased drug efficacy.
PB Elsevier
SN 1742-7061
YR 2014
FD 2014
LK https://uvadoc.uva.es/handle/10324/82818
UL https://uvadoc.uva.es/handle/10324/82818
LA eng
NO Acta Biomaterialia Volume 10, Issue 6, June 2014, Pages 2653-2662
DS UVaDOC
RD 18-feb-2026