RT info:eu-repo/semantics/article
T1 Surface Plasmon Resonance Imaging by Holographic Enhanced Mapping
A1 Mandracchia, B.
A1 Pagliarulo, V.
A1 Paturzo, M.
A1 Ferraro, P.
AB We designed, constructed and tested a holographic surface plasmon resonance (HoloSPR) objective-based microscope for simultaneous amplitude-contrast and phase-contrast surface plasmon resonance imaging (SPRi). SPRi is a widely spread tool for label-free detection of changes in refractive index and concentration, as well as mapping of thin films. Currently, most of the SPR sensors rely on the detection of amplitude or phase changes of light. Despite the high sensitivities achieved so far, each technique alone has a limited detection range with optimal sensitivity. Here we use a high numerical aperture objective that avoids all the limitations due to the use of a prism-based configuration, yielding highly magnified and distortion-free images. Holographic reconstructions of SPR images and real-time kinetic measurements are presented to show the capability of HoloSPR to provide a versatile imaging method for high-throughput SPR detection complementary to conventional SPR techniques.
SN 0003-2700
YR 2015
FD 2015
LK https://uvadoc.uva.es/handle/10324/82979
UL https://uvadoc.uva.es/handle/10324/82979
LA spa
NO Analitical Chemistry 2015 87, 4124–4128
DS UVaDOC
RD 23-feb-2026