RT info:eu-repo/semantics/article
T1 Holographic microscope slide in a spatio-temporal imaging modality for reliable 3D cell counting
A1 Mandracchia, Biagio
A1 Bianco, Vittorio
A1 Wang, Zhe
A1 Mugnano, Martina
A1 Bramanti, Alessia
A1 Paturzo, Melania
A1 Ferraro, Pietro
AB A Lab-on-a-Chip device for space-time digital holography is presented. Here, computational methods, holography, and microfluidics are intertwined to provide a reliable system for high-throughput counting of RBCs.In the current trend of miniaturization and simplification of imaging flow cytometry, Lab-on-a-Chip (LoC) microfluidic devices represent an innovative and cost-effective solution. In this framework, we propose for the first time a novel platform based on the compactness of a holographic microscope slide (HMS) in combination with the new computational features of space-time digital holography (STDH) that uses a 1D linear sensor array (LSA) instead of 2D CCD or CMOS cameras to respond to real diagnostic needs. In this LoC platform, computational methods, holography, and microfluidics are intertwined in order to provide an imaging system with a reduced amount of optical components and capability to achieve reliable cell counting even in the absence of very accurate flow control. STDH exploits the sample motion into the microfluidic channel to obtain an unlimited field-of-view along the flow direction, independent of the magnification factor. Furthermore, numerical refocusing typical of a holographic modality allows imaging and visualization of the entire volume of the channel, thus avoiding loss of information due to the limited depth of focus of standard microscopes. Consequently, we believe that this platform could open new perspectives for enhancing the throughput by 3D volumetric imaging.
SN 1473-0197
YR 2017
FD 2017
LK https://uvadoc.uva.es/handle/10324/82981
UL https://uvadoc.uva.es/handle/10324/82981
LA eng
NO Lab on a Chip 2017 17, 2831–2838
DS UVaDOC
RD 23-feb-2026