RT info:eu-repo/semantics/article T1 Storage temperature and UV-irradiation influence on the ergosterol content in edible mushrooms A1 Villares, Ana A1 Mateo-Vivaracho, Laura A1 García-Lafuente, Ana A1 Guillamón, Eva K1 Ergosterol K1 Ergosteryl ester K1 Mushroom K1 Fungi K1 HPLC AB Ergosterol (5,7,22-ergostatrien-3β-ol) and ergosteryl derivatives from different genera of edible mushrooms were separated and quantified by an isocratic reversed-phase high performance liquid chromatography (HPLC) method. The technique allowed a rapid separation of free ergosterol and two ergosteryl derivatives occurring in mushrooms. The ergosterol content varied considerably depending on the fungus. Thus, the species Agaricus bisporus and Hygrophorus marzuolus presented high quantities of ergosterol (6.4–6.8 mg/g, dry matter) followed by Pleurotus ostreatus, Calocybe gambosa, Lentinus edodes, and Boletus edulis (3.3–4.0 mg/g). In contrast, other species, such as Cantharellus cibarius, Lactarius deliciosus and Craterellus cornucopioides, contained significantly lower ergosterol amounts (0.2–0.4 mg/g). Two ergosteryl derivatives were found in mushrooms and also the content depended on the fungus. The stability of ergosterol, in terms of the formation of ergosterol peroxide, was evaluated under different storage temperatures and UV radiation. The lower the temperature (−20 °C) and the radiation time (10 min), the lower ergosterol oxidation was observed. PB Elsevier SN 0308-8146 YR 2014 FD 2014 LK https://uvadoc.uva.es/handle/10324/83137 UL https://uvadoc.uva.es/handle/10324/83137 LA eng NO Food Chemistry Volume 147, 15 March 2014, Pages 252-256 DS UVaDOC RD 27-feb-2026