RT info:eu-repo/semantics/article
T1 Integrated biorefinery scheme for apple pomace: Synergistic extraction and fermentation for dual recovery of pectin and 2,3-butanediol
A1 Lozano Martín, Alberto
A1 González Galán, Alba Mei
A1 López Linares, Juan Carlos
A1 Coca Sanz, Mónica
A1 García Cubero, María Teresa
A1 Lucas Yagüe, Susana
K1 Apple pomace
K1 Microwave-Assisted Extraction
K1 Ultrafiltration
K1 Pectin
K1 2,3-butanediol
K1 3308 Ingeniería y Tecnología del Medio Ambiente
AB This study presents an integrated, solvent-free biorefinery approach that couples microwave-assisted extraction(MAE), ultrafiltration (UF), and separate enzymatic hydrolysis and fermentation (SHF) to co-produce pectin-derived oligosaccharides (POS), predominantly oligogalacturonides (OGalA), and 2,3-butanediol (2,3-BDO) fromapple pomace (AP). Through response surface methodology, optimized MAE temperature and time conditions tomaximize OGalA formation were identified, while also limiting sugar degradation. Under these conditions(136 ◦C, 8.1 min), the extract contained OGalA at 4.0 ± 0.2 g/L, corresponding to a recovery of 53.7 ± 0.8%, anda pectin yield of 22.3 ± 0.2% was achieved (dry AP basis). Subsequently, an ultrafiltration step using a 3 kDamembrane yielded an OGalA solution concentrated to 11.5 ± 0.1 g/L and partially depleted small molecules.Compositional profiling also confirmed a notable enrichment of the rest of the oligosaccharide fractions, withincreases ranging from 2.4- to 3.2-fold. In parallel, the MAE-pretreated solid, selectively depleted in pectic do-mains and enriched in glucan (~37%), was saccharified and fermented via a sequential hydrolysis-fermentationscheme to produce 2,3-BDO. Fermentation trials with Bacillus strains achieved concentrations of 10.3 ± 0.4 g/L(B. amyloliquefaciens) and 9.5 ± 0.2 g/L within 47 h (B. licheniformis), with sugar clearance below 48 h at10–15% (w/v) solids loading. In conclusion, an integrated MAE → UF → SHF configuration co-producing POSand 2,3-BDO offers a practical route to valorize AP and advance circular bioeconomy goals in fruit-processingregions.
PB Elsevier
SN 2589-014X
YR 2026
FD 2026
LK https://uvadoc.uva.es/handle/10324/83307
UL https://uvadoc.uva.es/handle/10324/83307
LA eng
NO Bioresource Technology Reports, 2026, vol. 34, p. 102638
NO Producción Científica
DS UVaDOC
RD 04-mar-2026