RT info:eu-repo/semantics/article
T1 Innovative application of Nile Red (NR)-based dye for direct detection of micro and nanoplastics (MNPs) in diverse aquatic environments
A1 Peinador, R.I.
A1 H.P, Phuong Thanh
A1 Calvo, Jose I.
K1 Microplastics; nanoplastics; Nile Red staining; environment; fluorescence-fluorometry
AB This paper presents the results of a research aimed at establishing a novel method for the detection of primary and secondary micro- and nanoplastics (MNPs), by using the fluorescence properties of the dye Nile Red-n-heptane (NR-H). The method has been applied to the detection of laboratory degraded polymers (Polystyrene, PS and Polyethylene Terephthalate, PET) as well as traceable latex microspheres in aqueous environments, showing a remarkable detection capacity and avoiding the prior extraction or processing of MNPs in natural samples, with significant time savings compared to conventional methods. The study has been carried out on various types of water, including samples from wastewater treatment plants, boreholes, seawater and synthesized seawater. The effectiveness of the staining process was evaluated by scanning electron microscopy (SEM), dynamic light scattering (DLS) and optical microscopy. As a result, a novel standardizable protocol for the rapid detection of MNPs has been established, with the potential to improve environmental protection through fast in-situ detection and identification of plastic contaminants. The limitations of the protocol in the quantification of MNPs have also been identified and further studies are proposed to overcome these limitations.
PB Elsevier B.V.
SN 0045-6535
YR 2024
FD 2024
LK https://uvadoc.uva.es/handle/10324/83393
UL https://uvadoc.uva.es/handle/10324/83393
LA spa
NO Chemosphere, Volume 362, 2024, 142609
NO Producción Científica
DS UVaDOC
RD 11-mar-2026