RT info:eu-repo/semantics/article
T1 Influence of biomilking on methanotrophs cultivation during biogas conversion into ectoines
A1 Torres Franco, Andrés Felipe
A1 Herrero Lobo, Raquel
A1 Lebrero Fernández, Raquel
A1 Muñoz Torre, Raúl
AB Ectoine is one of the most profitable value chains for biogas valorisation. This study assessed the long-term effects of biomilking for ectoine (Ect) and hydroxyectoine (Hy) extraction on upstream methane bioconversion into ectoines using a halotolerant methanotrophic consortium cultivated in a Taylor-Flow bioreactor. After a control stage (S-I), fractions of the culture broth volume of 10 %, 50 %, 30 % and 60 % (S-II to S-VI) were subjected to biomilking before a final control (S-VII). No adverse effects were observed at 10 %, while higher fractions led to salt depletion, a ∼10 % reduction in CH4 bioconversion efficiency, and a loss of dominant ectoine producers, mainly Methylomicrobium. A decrease in intracellular Ect was also observed. Restoring salt levels (S-VI) recovered Methylomicrobium dominance and Ect content in the culture broth. Enhanced biomilking yielded up to 27.2 g-Ect + Hy per inlet kg of CH4, supporting its feasibility for sustainable biogas valorisation at a commercial scale.
PB Elsevier
SN 0960-8524
YR 2026
FD 2026
LK https://uvadoc.uva.es/handle/10324/83816
UL https://uvadoc.uva.es/handle/10324/83816
LA spa
NO Bioresource Technology, Marzo 2026, vol. 444, p. 133920
NO Producción Científica
DS UVaDOC
RD 18-abr-2026