RT info:eu-repo/semantics/article
T1 Optimized Continuous Bioproduction of Ectoine from Carbon Dioxide and Industrial Contaminants Using Guyparkeria halophila
A1 Huang Lin, Elisa
A1 Cantera Ruiz De Pellon, Sara
A1 Lebrero Fernández, Raquel
K1 C1 valorization
K1 Extremolytes
K1 Green pharmaceutical synthesis
K1 Halophiles
K1 Thiosulfate-oxidation
K1 3308 Ingeniería y Tecnología del Medio Ambiente
AB The production of high-value compounds such as ectoine in the pharmaceutical industry faces significant challenges, including high costs, resource intensity and reliance on refined sugars. This study presents a novel bioproduction platform converting carbon dioxide (CO2) and the industrial contaminant thiosulfate (S2O32-) into ectoine using the halophilic strain Guyparkeria halophila. To overcome limitations in biomass accumulation and incomplete S2O32- oxidation, cultivation and operational parameters, including S2O32- loading rate, pH, and dilution rate, were systematically optimized in continuous stirred tank reactors. A S2O32- loading rate of 5 g d-1 supported higher specific ectoine accumulation and promoted complete S2O32- oxidation, while a moderate pH increase up to 7.6 further improved CO2 assimilation. Additionally, implementing a prior semi-batch operation followed by a low dilution rate stage (0.10 d-1) effectively enhanced biomass and ectoine productivity. Under these optimized conditions, biomass accumulation increased significantly to 290.0 ± 20.2 mg L-1, with specific ectoine contents of 387.3 ± 23.1 mgEct gbiomass-1 and productivities of 10.6 ± 0.6 gEct m-3 d-1. This work demonstrated a scalable, efficient and sustainable platform for ectoine biosynthesis that integrates CO2 valorization and industrial by-product utilization, highlighting the potential of halophilic microbes for greener and economically viable pharmaceutical manufacturing.
PB Elsevier
SN 2213-3437
YR 2026
FD 2026
LK https://uvadoc.uva.es/handle/10324/83838
UL https://uvadoc.uva.es/handle/10324/83838
LA eng
NO Journal of Environmental Chemical Engineering, 2026, p. 122338
NO Producción Científica
DS UVaDOC
RD 29-mar-2026