RT info:eu-repo/semantics/article
T1 Transcellular diapedesis is initiated by invasive podosomes
A1 Carman, Christopher V.
A1 Sage, Peter T.
A1 Sciuto, Tracey E.
A1 Fuente García, Miguel Ángel de la
A1 Geha, Raif S.
A1 Ochs, Hans D.
A1 Dvorak, Harold F.
A1 Dvorak, Ann M.
A1 Springer, Timothy A.
K1 Pie - Enfermedades
AB Diapedesis is critical for immune system function and inflammatory responses. This occurs by migration of blood leukocytes either directly through individual microvascular endothelial cells (the “transcellular” route) or between them (the “paracellular” route). Mechanisms for transcellular pore formation in endothelium remain unknown. Here we demonstrate that lymphocytes used podosomes and extended “invasive podosomes” to palpate the surface of, and ultimately form transcellular pores through, the endothelium. In lymphocytes, these structures were dependent on Src kinase and the actin regulatory protein WASP; inhibition of podosome formation selectively blocked the transcellular route of diapedesis. In endothelium, membrane fusion events dependent on the SNARE-containing membrane fusion complex and intracellular calcium were required for efficient transcellular pore formation in response to podosomes. These findings provide insights into basic mechanisms for leukocyte trafficking and the functions of podosomes.
PB Elsevier
SN 1074-7613
YR 2007
FD 2007
LK http://uvadoc.uva.es/handle/10324/9825
UL http://uvadoc.uva.es/handle/10324/9825
LA eng
NO Immunity. 2007 Jun; 26(6): 784–797
NO Producción Científica
DS UVaDOC
RD 24-nov-2024