RT info:eu-repo/semantics/article T1 3BP2 Deficiency impairs the response of B cells, but not T cells, to antigen receptor ligation A1 Fuente García, Miguel Ángel de la A1 Kumar, Lalit A1 Lu, Bao A1 Geha, Raif S. K1 Biología celular AB The adapter protein 3BP2 is expressed in lymphocytes; binds to Syk/ZAP-70, Vav, and phospholipase C-γ (PLC-γ); and is thought to be important for interleukin-2 gene transcription in T cells. To define the role of 3BP2 in lymphocyte development and function, we generated 3BP2-deficient mice. T-cell development, proliferation, cytokine secretion, and signaling in response to T-cell receptor (TCR) ligation were all normal in 3BP2−/− mice. 3BP2−/− mice had increased accumulation of pre-B cells in the bone marrow and a block in the progression of transitional B cells in the spleen from the T1 to the T2 stage, but normal numbers of mature B cells. B-cell proliferation, cell cycle progression, PLC-γ2 phosphorylation, calcium mobilization, NF-ATp dephosphorylation, and Erk and Jnk activation in response to B-cell receptor (BCR) ligation were all impaired. These results suggest that 3BP2 is important for BCR, but not for TCR signaling. PB American Society for Microbiology SN 0270-7306 YR 2006 FD 2006 LK http://uvadoc.uva.es/handle/10324/9828 UL http://uvadoc.uva.es/handle/10324/9828 LA eng NO Molecular and Cellular Biology, 2006, vol. 26, n. 14. p. 5214–5225 NO Producción Científica DS UVaDOC RD 06-dic-2024