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Título
Novel haloalkaliphilic methanotrophic bacteria: An attempt for enhancing methane bio-refinery
Autor
Año del Documento
2019
Editorial
Elsevier
Descripción
Producción Científica
Documento Fuente
Journal of Environmental Management, 2019, Volume 231, Pages 1091-1099
Zusammenfassung
Methane bioconversion into products with a high market value, such as ectoine or hydroxyectoine, can be optimized via isolation of more efficient novel methanotrophic bacteria. The research here presented focused on the enrichment of methanotrophic consortia able to co-produce different ectoines during CH4 metabolism. Four different enrichments (Cow3, Slu3, Cow6 and Slu6) were carried out in basal media supplemented with 3 and 6% NaCl, and using methane as the sole carbon and energy source. The highest ectoine accumulation (∼20 mg ectoine g biomass−1) was recorded in the two consortia enriched at 6% NaCl (Cow6 and Slu6). Moreover, hydroxyectoine was detected for the first time using methane as a feedstock in Cow6 and Slu6 (∼5 mg g biomass−1). The majority of the haloalkaliphilic bacteria identified by 16S rRNA community profiling in both consortia have not been previously described as methanotrophs. From these enrichments, two novel strains (representing novel species) capable of using methane as the sole carbon and energy source were isolated: Alishewanella sp. strain RM1 and Halomonas sp. strain PGE1. Halomonas sp. strain PGE1 showed higher ectoine yields (70–92 mg ectoine g biomass−1) than those previously described for other methanotrophs under continuous cultivation mode (∼37–70 mg ectoine g biomass−1). The results here obtained highlight the potential of isolating novel methanotrophs in order to boost the competitiveness of industrial CH4-based ectoine production.
Palabras Clave
Metano
Methane
ISSN
0301-4797
Revisión por pares
NO
Patrocinador
Ministerio de Economía, Industria y Competitividad (Project CTM2015-70442-R project and Red NOVEDAR)
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. UIC71)
European Union through the FEDER Funding Program (PhD Grant contract Nº E-47-2014-0140696)
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. UIC71)
European Union through the FEDER Funding Program (PhD Grant contract Nº E-47-2014-0140696)
Version del Editor
Idioma
eng
Derechos
openAccess
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