Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/35202
Título
Measuring Ca2 + inside intracellular organelles with luminescent and fluorescent aequorin-based sensors
Autor
Año del Documento
2017
Editorial
Elsevier
Descripción
Producción Científica
Documento Fuente
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Volume 1864, Issue 6, 2017, Pages 894-899
Abstract
GFP-Aequorin Protein (GAP) can be used to measure [Ca2 +] inside intracellular organelles, both by luminescence and by fluorescence. The low-affinity variant GAP3 is adequate for ratiometric imaging in the endoplasmic reticulum and Golgi apparatus, and it can be combined with conventional synthetic indicators for simultaneous measurements of cytosolic Ca2 +. GAP is bioorthogonal as it does not have mammalian homologues, and it is robust and functionally expressed in transgenic flies and mice, where it can be used for Ca2 + measurements ex vivo and in vivo to explore animal models of health and disease. This article is part of a Special Issue entitled: ECS Meeting edited by Claus Heizmann, Joachim Krebs and Jacques Haiech.
Palabras Clave
Aequorina
Aequorin
Revisión por pares
SI
Patrocinador
Ministerio de Economía, Industria y Competitividad (Project BFU2014-53469P)
Instituto de Salud Carlos III (TerCel; RD16/0011/0003)
Instituto de Salud Carlos III (TerCel; RD16/0011/0003)
Version del Editor
Idioma
eng
Derechos
openAccess
Collections
Files in this item
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International