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Título
Recovery of proteins from biomass grown in pig manure microalgae-based treatment plants by alkaline hydrolysis and acidic precipitation
Autor
Año del Documento
2019
Editorial
Elsevier
Descripción
Producción Científica
Documento Fuente
Bioresource Technology, 2019, vol. 273. p. 599-607
Résumé
The influence of three variables on key parameters of the protein extraction process (an alkaline hydrolysis followed by an acidic precipitation) for biomass from innovative photo-bioreactors for pig manure treatment was evaluated. Alkaline hydrolysis provided high solubilisation values (up to 66.5% of the biomass), augmenting with increasing values of the three studied variables (NaOH concentration, temperature and time). Nevertheless, moderate total (13.2%) and protein extraction yields (16.9%) were obtained, which was attributable to protein denaturation or to the low effectivity of the precipitation method. Extracts rich in proteins (53.5%–77.9%) with suitable amino acid profiles were obtained, but significant amounts of the initial lipids (up to 44.6%) were co-extracted probably due to fatty acids saponification. These results establish the first step for future studies in enhancing cell wall disruption and protein recovery by coupling alkaline hydrolysis with other mechanical pre-treatments, while considering alternative separation and purification methods.
Palabras Clave
Protein extraction
Extracción de proteínas
Lipids
Lípidos
pH-shift
Variación de pH
Solubilisation
Solubilización
Wastewater
Aguas residuales
ISSN
0960-8524
Revisión por pares
SI
Patrocinador
Junta de Castilla y León (Projects UIC 071 / CLU 2017-09 / VA080G18)
Ministerio de Ciencia, Innovación y Universidades (Project CTQ2017-84006-C3-1-R)
EU-FEDER (Projects CLU 2017-09 / CTQ2017-84006-C3-1-R)
Ministerio de Ciencia, Innovación y Universidades (Project CTQ2017-84006-C3-1-R)
EU-FEDER (Projects CLU 2017-09 / CTQ2017-84006-C3-1-R)
Version del Editor
Propietario de los Derechos
© 2019 Elsevier
Idioma
eng
Tipo de versión
info:eu-repo/semantics/submittedVersion
Derechos
openAccess
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