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Título
Transferability of PCR-based diagnostic protocols: An international collaborative case study assessing protocols targeting the quarantine pine pathogen Fusarium circinatum
Autor
Año del Documento
2019
Editorial
Springer Nature
Descripción
Producción Científica
Documento Fuente
Scientific Reports, 2019, vol. 9. 17 p.
Résumé
Fusarium circinatum is a harmful pathogenic fungus mostly attacking Pinus species and also
Pseudotsuga menziesii, causing cankers in trees of all ages, damping-of in seedlings, and mortality in
cuttings and mother plants for clonal production. This fungus is listed as a quarantine pest in several
parts of the world and the trade of potentially contaminated pine material such as cuttings, seedlings or
seeds is restricted in order to prevent its spread to disease-free areas. Inspection of plant material often
relies on DNA testing and several conventional or real-time PCR based tests targeting F. circinatum are
available in the literature. In this work, an international collaborative study joined 23 partners to assess
the transferability and the performance of nine molecular protocols, using a wide panel of DNA from 71
representative strains of F. circinatum and related Fusarium species. Diagnostic sensitivity, specifcity
and accuracy of the nine protocols all reached values >80%, and the diagnostic specifcity was the only
parameter difering signifcantly between protocols.The rates of false positives and of false negatives
were computed and only the false positive rates difered signifcantly, ranging from 3.0% to 17.3%.The
diference between protocols for some ofthe performance values were mainly due to cross-reactions
with DNA from non-target species, which were either not tested or documented in the original articles.
Considering that participating laboratories were free to use their own reagents and equipment, this
study demonstrated that the diagnostic protocols for F. circinatum were not easily transferable to endusers. More generally, our results suggest that the use of protocols using conventional or real-time PCR
outside their initial development and validation conditions should require careful characterization of
the performance data priorto use under modifed conditions (i.e. reagents and equipment). Suggestions
to improve the transfer are proposed.
Materias Unesco
3106 Ciencia Forestal
Palabras Clave
Pine pitch canker
Chancro resinoso del pino
PCR-based tests
Tests PCR
Diagnóstico de enfermedad fungica
Fungal infections - Diagnosis
Enfermedades fúngicas - Diagnóstico
ISSN
2045-2322
Revisión por pares
SI
Patrocinador
European Cooperation in Science and Technology (COST Action FP1406 PINESTRENGTH)
Estonian Science Foundation (grants PSG136 and IUT21-04)
Centro de Estudios do Ambiente e do Mar (CESAM) (projects UID/AMB/50017/2013 and POCI-01- 0145- FEDER-007638)
Slovenian Research Agency (project P4-0107)
Estonian Science Foundation (grants PSG136 and IUT21-04)
Centro de Estudios do Ambiente e do Mar (CESAM) (projects UID/AMB/50017/2013 and POCI-01- 0145- FEDER-007638)
Slovenian Research Agency (project P4-0107)
Version del Editor
Propietario de los Derechos
© 2019 Springer Nature
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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