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Título
Rapid changes in anterior pituitary cell phenotypes in male and female mice after acute cold stress
Autor
Año del Documento
2008
Editorial
Oxford University Press
Descripción
Producción Científica
Documento Fuente
Endocrinology, 2008, vol. 149, n. 5. p. 2159–2167
Zusammenfassung
The anterior pituitary (AP) is made of five different cell types. The relative abundance and phenotype of AP cells may change in different physiological situations as an expression of pituitary plasticity. Here, we analyze in detail the phenotype of mouse corticotropes and the effects of acute cold stress on AP cell populations. The hormone content and the expression of hypothalamic-releasing hormone (HRH) receptors in all the five AP cell types were studied in the male and female mice at rest and after a 30-min cold stress. Expression of HRH receptors was evidenced by imaging the single-cell cytosolic Ca2+ responses in fura-2-loaded cells. Hormone contents were studied by multiple, simultaneous immunofluorescence of all the five hormones. Corticotropes displayed a striking sexual dimorphism, even in the resting condition. Male corticotropes showed the orthodox phenotype. They were monohormonal, storing only ACTH, and monoreceptorial, responding only to CRH. In contrast, female corticotropes were made of about equal parts of orthodox cells and multifunctional cells, which co-stored additional AP hormones and expressed additional HRH receptors. Cold stress did not modify the number of ACTH containing cells, but, according to immunostaining, it increased the relative abundance of other AP cell types at the expense of the pool of cells storing no hormones. Cold stress also modified the response to CRH and other HRHs. Most of these phenotypical changes presented a strong sexual dimorphism. These results indicate that pituitary plasticity is even larger than previously thought.
Palabras Clave
Pituitary Cells
Células pituirarias
Phenotyes
Fenotipos
Mice
Ratones
Cold stress
Estrés por frío
ISSN
1945-7170
Revisión por pares
SI
Patrocinador
Fondo de Investigaciones Sanitarias (grant FIS03/1231)
Ministerio de Ciencia, Innovación y Universidades (grants BFU-2004-02765/BFI, BFU2005-02078 and BFU2007-60157)
Ministerio de Ciencia, Innovación y Universidades (grants BFU-2004-02765/BFI, BFU2005-02078 and BFU2007-60157)
Version del Editor
Propietario de los Derechos
© 2008 Oxford University Press
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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