Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/45091
Título
Effects of UVA+UVB solar radiation levels and cross-linking on animal model with Bowman’s layer: Chicken
Autor
Año del Documento
2019
Editorial
Herald Scholarly
Descripción
Producción Científica
Documento Fuente
Journal of Ophthalmology & Clinical Research, 2019, vol. 6, n. 3. 8 p.
Abstract
Purpose: Evaluate the corneal damage and wound healing in an animal model with Bowman’s layer after UVA+UVB radiation and epioff Cross-Linking (CXL). Methods: Adult Brown Classic chickens were randomly divided into three groups. The UVA+UVB group (20 eyes) was exposed to three doses of radiation (4.60 J/cm2) . The CXL group (12 eyes), was de-epithelialized and irradiated with UVA light (5.4 J/cm2) for 30 minutes. The contralateral eyes were used as a control (12 eyes). The animals have clinically follow-up, the epithelial closure was measured by fluorescein stain and corneal thickness by pachymetry. Keratocytes loss, subsequent cell repopulation, and healing events were analyzed after euthanasia at 24 hours, 15 and 30 days post-radiation on hematoxylin-eosin-stained sections. Cell death was detected by TUNEL assay, proliferation by BrdU (bromodeoxyuridine) immunofluorescence, and myofibroblasts differentiation by (αSMA) immunohistochemistry. Results: Re-epithelialization was at 5 ± 2.3 days in CXL and at day 2 ± 0.9 days in UVA+UVB. Pachymetry was higher after UVA+UVB than CXL (p<0.05). Cell death affected the whole thickness of stroma in the UVA+UVB group and anterior and middle stroma in CXL. At day 1, the UVA+UVB group showed a more significant reduction in the number of stromal cells. After 30 days, both groups showed a free band of cells below the epithelium. Conclusion: The damage and the healing response in chicken corneas after UVA+UVB and CXL demonstrated that Bowman’s layer has not been successful in protecting the cornea from radiation. Perhaps, other factors that are involved in corneal defense.
Materias Unesco
2209.15 Optometría
Palabras Clave
Bowman’s layer
Membrana de Bowman
Córnea
Cross-linking technique
Técnica de cross-linking
Ultraviolet radiation
Radiación ultravioleta
ISSN
2378-8887
Revisión por pares
SI
Version del Editor
Propietario de los Derechos
© 2019 Herald Scholarly
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
Aparece en las colecciones
Files in questo item
![Attribution-NonCommercial-NoDerivatives 4.0 Internacional](/themes/Mirage2//images/creativecommons/cc-by-nc-nd.png)