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Título
Assessment of the corneal collagen organization after chemical burn using second harmonic generation microscopy
Autor
Año del Documento
2021
Editorial
Optical Society of America (OSA)
Descripción
Producción Científica
Documento Fuente
Biomedical Optics Express Vol. 12, Issue 2, pp. 756-765 (2021)
Abstract
The organization of the corneal stoma is modified due to different factors, including pathology, surgery or external damage. Here the changes in the organization of the corneal collagen fibers during natural healing after chemical burn are investigated using second harmonic generation (SHG) imaging. Moreover, the structure tensor (ST) was used as an objective tool for morphological analyses at different time points after burn (up to 6 months). Unlike control corneas that showed a regular distribution, the collagen pattern at 1 month of burn presented a non-organized arrangement. SHG signal levels noticeably decreased and individual fibers were hardly visible. Over time, the healing process led to a progressive re-organization of the fibers that could be quantified through the ST. At 6 months, the stroma distribution reached values similar to those of control eyes and a dominant direction of the fibers re-appeared. The present results show that SHG microscopy imaging combined with the ST method is able to objectively monitor the temporal regeneration of the corneal organization after chemical burn. Future implementations of this approach into clinically adapted devices would help to diagnose and quantify corneal changes, not only due to chemical damages, but also as a result of disease or surgical procedures.
Materias (normalizadas)
Córnea - cirugía
Materias Unesco
2407 Biología Celular
Palabras Clave
Colágeno corneal
Corneal collagen
ISSN
2156-7085
Revisión por pares
SI
Patrocinador
Ministerio de Sanidad. Instituto de Salud Carlos III (PI15/01906)
Fundación Séneca (19897/GERM/15)
Fundación Séneca (19897/GERM/15)
Nota
© 2021 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
Version del Editor
Propietario de los Derechos
© 2021 Optical Society of America
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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