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Título
The Incorporation of etanercept into a porous tri-layer scaffold for restoring and repairing cartilage tissue
Autor
Año del Documento
2022
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Pharmaceutics, 2022, vol. 14, n. 2, 282
Résumé
Cartilage diseases currently affect a high percentage of the world’s population. Almost
all of these diseases, such as osteoarthritis (OA), cause inflammation of this soft tissue. However,
this could be controlled with biomaterials that act as an anti-inflammatory delivery system, capable
of dosing these drugs over time in a specific area. The objective of this study was to incorporate
etanercept (ETA) into porous three-layer scaffolds to decrease the inflammatory process in this soft
tissue. ETA is a blocker of pro-inflammatory cytokines, such as tumour necrosis factor alpha (TNF-α)
and interleukin 6 (IL-6). For this reason, the scaffold was built based on natural polymers, including
chitosan and type I collagen. The scaffold was grafted next to subchondral bone using hydroxyapatite
as filler. One of the biomaterials obtained was also crosslinked to compare its mechanical properties
with the non-treated one. Both samples’ physicochemical properties were studied with SEM, microCT and photoacoustic imaging, and their rheological properties were also compared. The cell viability
and proliferation of the human chondrocyte C28/I2 cell line were studied in vitro. An in vitro and
in vivo controlled release study was evaluated in both specimens. The ETA anti-inflammatory effect
was also studied by in vitro TNF-α and IL-6 production. The crosslinked and non-treated scaffolds
had rheological properties suitable for this application. They were non-cytotoxic and favoured the
in vitro growth of chondrocytes. The in vitro and in vivo ETA release showed desirable results for a
drug delivery system. The TNF-α and IL-6 production assay showed that this drug was effective
as an anti-inflammatory agent. In an in vivo OA mice model, safranin-O and fast green staining
was carried out. The OA cartilage tissue improved when the scaffold with ETA was grafted in the
damaged area. These results demonstrate that this type of biomaterial has high potential for clinical
applications in tissue engineering and as a controlled drug delivery system in OA articular cartilage.
Palabras Clave
Osteoarthritis
Osteoartritis
Tissue engineering
Ingeniería de tejidos
Etanercept
Cartilage - Implants
Cartílago - Implantes
ISSN
1999-4923
Revisión por pares
SI
Patrocinador
European Union through the Erasmus PLUS doctoral fellowship (project 2015-1-NL01-KA 107-008639)
VIDI personal grant (project 723.012.110)
VIDI personal grant (project 723.012.110)
Patrocinador
info:eu-repo/grantAgreement/EC/H2020/644373
info:eu-repo/grantAgreement/EC/H2020/777682
info:eu-repo/grantAgreement/EC/H2020/807281
info:eu-repo/grantAgreement/EC/H2020/852985
info:eu-repo/grantAgreement/EC/H2020/734684
info:eu-repo/grantAgreement/EC/H2020/955335
info:eu-repo/grantAgreement/EC/H2020/952520
info:eu-repo/grantAgreement/EC/H2020/872391
info:eu-repo/grantAgreement/EC/H2020/860173
info:eu-repo/grantAgreement/EC/H2020/675743
info:eu-repo/grantAgreement/EC/H2020/861190
info:eu-repo/grantAgreement/EC/H2020/857894
info:eu-repo/grantAgreement/EC/H2020/859908
info:eu-repo/grantAgreement/EC/H2020/872860
info:eu-repo/grantAgreement/EC/H2020/777682
info:eu-repo/grantAgreement/EC/H2020/807281
info:eu-repo/grantAgreement/EC/H2020/852985
info:eu-repo/grantAgreement/EC/H2020/734684
info:eu-repo/grantAgreement/EC/H2020/955335
info:eu-repo/grantAgreement/EC/H2020/952520
info:eu-repo/grantAgreement/EC/H2020/872391
info:eu-repo/grantAgreement/EC/H2020/860173
info:eu-repo/grantAgreement/EC/H2020/675743
info:eu-repo/grantAgreement/EC/H2020/861190
info:eu-repo/grantAgreement/EC/H2020/857894
info:eu-repo/grantAgreement/EC/H2020/859908
info:eu-repo/grantAgreement/EC/H2020/872860
Version del Editor
Propietario de los Derechos
© 2022 The Authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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