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Título
Flow cytometry to assess the counts and physiological state of cronobacter sakazakii cells after heat exposure
Autor
Año del Documento
2019
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Foods, 2019, vol. 8, n. 12, 688
Résumé
Cronobacter sakazakii is an opportunistic pathogen that is associated with outbreaks of neonatal necrotizing enterocolitis, septicaemia, and meningitis. Reconstituted powdered infant formulae is the most common vehicle of infection. The aim of the present study is to gain insight into the physiological states of C. sakazakii cells using flow cytometry to detect the compromised cells, which are viable but non-culturable using plate-based methods, and to evaluate the impact of milk heat treatments on those populations. Dead-cell suspensions as well as heat-treated and non-heat-treated cell suspensions were used. After 60 or 65 °C treatments, the number of compromised cells increased as a result of cells with compromised membranes shifting from the heat-treated suspension. These temperatures were not effective at killing all bacteria but were effective at compromising their membranes. Thus, mild heat treatments are not enough to guarantee the safety of powered infant formulae. Flow cytometry was capable of detecting C. sakazakii’s compromised cells that cannot be detected with classical plate count methods; thus, it could be used as a screening test to decrease the risk derived from the presence of pathogenic viable but non-culturable cells in this food that is intended for newborns’ nutrition.
Palabras Clave
Cytometry
Citometría
Cronobacter sakazakii
ISSN
2304-8158
Revisión por pares
SI
Patrocinador
Junta de Castilla y León (projects SAN196/VA07/07, SAN673/VA05/08, and SAN126/09)
Version del Editor
Propietario de los Derechos
© 2019 The Authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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