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Título
Amperometric biosensor for oxalate determination in urine using sequential injection analysis
Autor
Año del Documento
2012
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Molecules, 2012, vol. 17, n. 8, p. 8859-8871
Abstract
An amperometric flow biosensor for oxalate determination in urine samples after enzymatic reaction with oxalate oxidase immobilized on a modified magnetic solid is described. The solid was magnetically retained on the electrode surface of an electrode modified with Fe (III)-tris-(2-thiopyridone) borate placed into a sequential injection system preceding the amperometric detector. The variables involved in the system such as flow rate, aspired volumes (modified magnetic suspension and sample) and reaction coil length were evaluated using a Taguchi parameter design. Under optimal conditions, the calibration curve of oxalate was linear between 3.0–50.0 mg·L−1, with a limit of detection of 1.0 mg·L−1. The repeatability for a 30.0 mg·L−1 oxalate solution was 0.7%. The method was validated by comparing the obtained results to those provided by the spectrophotometric method; no significant differences were observed.
Materias Unesco
23 Química
2301 Química Analítica
Palabras Clave
Oxalate
Urine
Sequential injection analysis
Magnetic particles
ISSN
1420-3049
Revisión por pares
SI
Patrocinador
CONACyT (Project 178607)
Ministerio de Ciencia e Innovación (MCINN AN2010-00234)
Junta de Castilla y León (Project VA023A10-2)
Ministerio de Ciencia e Innovación (MCINN AN2010-00234)
Junta de Castilla y León (Project VA023A10-2)
Version del Editor
Propietario de los Derechos
© 2012 The Author(s)
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
Collections
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