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Título
Embryonic Cerebrospinal Fluid Collaborates With the Isthmic Organizer To Regulate Mesencephalic Gene Expression
Autor
Año del Documento
2005
Editorial
Willey InterScience
Descripción
Producción Científica
Documento Fuente
Journal of Neuroscience Research, 2005, vol. 82. p.333-345
Zusammenfassung
Early in development, the behavior of neuroepithelial
cells is controlled by several factors acting in a developmentally
regulated manner. Recently it has been
shown that diffusible factors contained within embryonic
cerebrospinal fluid (CSF) promote neuroepithelial
cell survival, proliferation, and neurogenesis in mesencephalic
explants lacking any known organizing center.
In this paper, we show that mesencephalic and mesencephalic
þ isthmic organizer explants cultured only with
basal medium do not express the typically expressed
mesencephalic or isthmic organizer genes analyzed
(otx2 and fgf8, respectively) and that mesencephalic
explants cultured with embryonic CSF-supplemented
medium do effect such expression, although they
exhibit an altered pattern of gene expression, including
ectopic shh expression domains. Other trophic sources
that are able to maintain normal neuroepithelial cell
behavior, i.e., fibroblast growth factor-2, fail to activate
this ectopic shh expression. Conversely, the expression
pattern of the analyzed genes in mesencephalic þ isthmic
organizer explants cultured with embryonic cerebrospinal
fluid-supplemented medium mimics the
pattern for control embryos developed in ovo. We demonstrate
that embryonic CSF collaborates with the isthmic
organizer in regulation of the expression pattern of
some characteristic neuroectodermal genes during
early stages of central nervous system (CNS) development,
and we suggest that this collaboration is not
restricted to the maintenance of neuroepithelial cell
survival. Data reported in this paper corroborate the
hypothesis that factors contained within embryonic
CSF contribute to the patterning of the CNS during
early embryonic development.
Materias (normalizadas)
Líquidos orgánicos
Neurología
Cerebro
Embriología humana
ISSN
0360-4012
Revisión por pares
SI
Propietario de los Derechos
Willey InterScience
Idioma
eng
Derechos
openAccess
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