Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/59495
Título
Essential oil and hydrophilic antibiotic co-encapsulation in multiple lipid nanoparticles: proof of concept and in vitro activity against Pseudomonas aeruginosa
Autor
Año del Documento
2021
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Antibiotics, 2021, vol. 10, n. 11, 1300
Zusammenfassung
In the worldwide context of an impending emergence of multidrug-resistant bacteria, this research combined the advantages of multiple lipid nanoparticles (MLNs) and the promising therapeutic use of essential oils (EOs) as a strategy to fight the antibiotic resistance of three Pseudomonas aeruginosa strains with different cefepime (FEP) resistance profiles. MLNs were prepared by ultrasonication using glyceryl trioleate (GTO) and glyceryl tristearate (GTS) as a liquid and a solid lipid, respectively. Rosemary EO (REO) was selected as the model EO. REO/FEP-loaded MLNs were characterized by their small size (~110 nm), important encapsulation efficiency, and high physical stability over time (60 days). An assessment of the antimicrobial activity was performed using antimicrobial susceptibility testing assays against selected P. aeruginosa strains. The assays showed a considerable increase in the antibacterial property of REO-loaded MLNs compared with the effect of crude EO, especially against P. aeruginosa ATCC 9027, in which the minimum inhibitory concentration (MIC) value decreased from 80 to 0.6 mg/mL upon encapsulation. Furthermore, the incorporation of FEP in MLNs stabilized the drug without affecting its antipseudomonal activity. Thus, the ability to co-encapsulate an essential oil and a hydrophilic antibiotic into MLN has been successfully proved, opening new possibilities for the treatment of serious antimicrobial infections.
Materias (normalizadas)
Microbiology
Bacterias
Antibióticos
Materias Unesco
2414.04 Bacteriología
2414.01 Antibióticos
Palabras Clave
Multiple lipid nanoparticles
Antimicrobial activity
Pseudomonas aeruginosa
Nanopartículas lipídicas múltiples
Actividad antimicrobiana
Revisión por pares
SI
Patrocinador
Tunisian Ministry of HEducation and Scientific Research and by the Fundación General de la Universidad de Valladolid (PIP 063/147181)
Fondo de Innovación, Tecnología y Economía Circular (FITEC) e iNOVA4Health (UIDB/04462/2020)
Fondo de Innovación, Tecnología y Economía Circular (FITEC) e iNOVA4Health (UIDB/04462/2020)
Version del Editor
Propietario de los Derechos
© 2021 The Authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
Aparece en las colecciones
Dateien zu dieser Ressource
![Atribución 4.0 Internacional](/themes/Mirage2//images/creativecommons/cc-by.png)