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Título
Apolipoprotein D alters the early transcriptional response to oxidative stress in the adult cerebellum
Autor
Año del Documento
2011
Editorial
International Society for Neurochemistry
Descripción
Producción Científica
Documento Fuente
Journal of Neurochemistry, 2011, vol. 117, p. 949-960
Resumo
The lipocalin Apolipoprotein D (ApoD), known to protect the
nervous system against oxidative stress (OS) in model
organisms, is up-regulated early in the mouse brain in
response to the ROS generator paraquat. However, the processes
triggered by this up-regulation have not been explored.
We present here a study of the effect of ApoD on the early
transcriptional changes upon OS in the mouse cerebellum
using microarray profiling. ApoD-KO and transgenic mice
over-expressing ApoD in neurons are compared to wild-type
controls. In control conditions, ApoD affects the transcriptional
profile of neuron and oligodendrocyte-specific genes involved
in neuronal excitability, synaptic function, and myelin
homeostasis. When challenged with paraquat, the absence of
ApoD modifies the response of genes mainly related to OS
management and myelination. Interestingly, the over-expression
of ApoD in neurons almost completely abolishes the early
transcriptional response to OS. We independently evaluate
the expression of protein kinase Cd, a gene up-regulated by
OS only in the ApoD-KO cerebellum, and find it overexpressed
in cultured ApoD-KO primary astrocytes, which
points to a role for ApoD in astrocyte-microglia signaling. Our
results support the hypothesis that ApoD is necessary for a
proper response of the nervous system against physiological
and pathological OS.
Materias (normalizadas)
Nervioso, sistema - Fisiología
ISSN
0022-3042
Revisión por pares
SI
Idioma
eng
Derechos
openAccess
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