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Título
Simultaneous determination of betaines and other quaternary ammonium related compounds in bee pollen by hydrophilic interaction liquid chromatography-mass spectrometry
Autor
Año del Documento
2020
Editorial
Elsevier
Descripción
Producción Científica
Documento Fuente
Microchemical Journal, Mayo, 2020, vol. 157, p. 105000
Zusammenfassung
The objective of this work was to develop a new method to determine nine betaines and other quaternary
ammonium related compounds in bee pollen from different botanical origins; hydrophilic interaction liquid
chromatography coupled to mass spectrometry (HILIC-MS) was the technique employed. A quick and efficient sample treatment (average analyte recoveries were between 82% and 95% and no significant matrix effect on ionization was found), involving an extraction with an acetonitrile and water mixture (1:1, v/v), centrifugation, freezing with dry ice followed by dilution, was presented. Chromatographic analysis (15 min) was by means of a core–shell HILIC column and a mobile phase applied in gradient elution mode. The analytical characteristics of the method were evaluated and the data demonstrated that not only was it selective, but it also displayed a wide linearity range and good precision. Several bee pollen samples from different botanical origins were analyzed with the proposed methodology, and three betaines (betaine, choline and trigonelline) were detected in all of them in a wide range of concentrations (57–62236 mg/kg).
Palabras Clave
Bee pollen; Betaines; Core-shell columns; Hydrophilic interaction liquid chromatography; Mass spectrometry; Sample treatment
Revisión por pares
SI
Patrocinador
Este trabajo forma parte de los proyectos de investigación financiados por el Ministerio de Economía y Competitividad e INIA-FEDER (RTA2015-00013-C03-01 y 03).
Propietario de los Derechos
Elsevier
Idioma
eng
Tipo de versión
info:eu-repo/semantics/acceptedVersion
Derechos
openAccess
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